Plan apochromate 63 1.4 oil immersion objective

Manufactured by Zeiss

The Plan-Apochromate 63×/1.4 oil immersion objective is a high-performance optical component designed for advanced microscopy applications. It features a magnification of 63x and a numerical aperture of 1.4, which enables enhanced resolution and light-gathering capabilities. This objective is optimized for use with oil immersion techniques to provide exceptional image quality and contrast.

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Lab products found in correlation

Lsm 510 meta laser scanning microscope, by Zeiss (2 mentions)

Close spelling variants of this product

63× oil immersion objective (81 citations) Plan-Apochromate (2 citations)

3 protocols using plan apochromate 63 1.4 oil immersion objective

Intracellular Uptake of APIM Peptide in Yeast

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Example 2

Yeast cells (Saccharomyces cerevisiae) were grown in LB media. A fluorescently labelled APIM peptide (ATX-101-FAM) was added to the media and incubated for 1-2 minutes before an aliquot of cells was removed and analyzed on a Zeiss LSM 510 Meta laser scanning microscope equipped with a Plan-Apochromate 63×/1.4 oil immersion objective.

FIG. 3 shows that ATX-101 is imported into yeast cells, thereby demonstrating that it must be acting intracellularly, i.e. unlike many antimicrobial compounds ATX-101 is not antimicrobial due to its effects on cell walls or membranes, i.e. it does not function by permeabilizing cells.

US10450348B2. Antimicrobial agents and their use in therapy (2019-10-22). Norwegian University of Science and Technology [NO]. Inventors: Marit Otterlei [NO], Siri Bachke [NO].

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Visualizing Intracellular Trafficking of APIM Peptide

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Example 2

US11337427B2. Antimicrobial agents and their use in therapy (2022-05-24). Norwegian University of Science and Technology [NO]. Inventors: Marit Otterlei [NO], Siri Bachke [NO].

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Fluorescent Yeast Cellular Imaging

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The fluorescently labeled APIM‐peptide (APIM‐peptide‐FAM) was added to yeast cells resuspended in phosphate‐buffered saline. The fluorescent live images were acquired 2–5 min after addition, using a Zeiss LSM 510 Meta laser scanning microscope equipped with a Plan‐Apochromate 63 × /1.4 oil immersion objective, excitation λ = 488 nm, and detection λ = 505–530 nm.

Olaisen C., Kvitvang H.F., Lee S., Almaas E., Bruheim P., Drabløs F, & Otterlei M. (2018). The role of PCNA as a scaffold protein in cellular signaling is functionally conserved between yeast and humans. FEBS Open Bio, 8(7), 1135-1145.

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