Sligrl nh2

Manufactured by Merck Group

Sourced in Germany, United States

SLIGRL-NH2 is a synthetic peptide. It functions as a selective protease-activated receptor 2 (PAR2) agonist. The core function of this peptide is to activate PAR2 receptors in experimental settings.

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Lab products found in correlation

6 protocols using sligrl nh2

Kreb's Solution for Cell Culture

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The Kreb’s solution contained (in mM, pH 7.2–7.4): 119 Na⁺, 4.7 K⁺, 1.2 H₂PO₄⁻, 25 HCO₃⁻, 1.2 Mg²⁺, 2.5 Ca²⁺ and 11.1 Glucose, and all the reagents were from Sinopharm Chemical Reagent Co., Ltd, China. Human/rat corticotropin releasing factor (CRF) was acquired from R&D Systems Inc., MN, USA. Cromolyn sodium, compound 48/80, aprotinin, nafamostat mesylate (FUT-175), ENMD-1068, SLIGRL-NH2, FD4 and FD40 were obtained from Sigma-Aldrich Co., LLC, USA.

Zhang L., Song J., Bai T., Qian W, & Hou X.H. (2017). Stress induces more serious barrier dysfunction in follicle-associated epithelium than villus epithelium involving mast cells and protease-activated receptor-2. Scientific Reports, 7, 4950.

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Synthesis of Loxapine Derivatives

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Loxapine, pregabalin, chloroquine, SLIGRL‐NH₂, β‐alanine, histamine, PGE₂, serotonin, and bradykinin were purchased from Sigma–Aldrich (Munich, Germany). All indicated concentrations refer to those of pure substances. Reagents and solvents for the synthesis of Loxapine derivatives were obtained from Acros Organics (Gel, Belgium), Alfa Aesar GmbH & Co KG (Karlsruhe, Germany), BLDPharm Inc. (NuiNan, China), Fluorochem Ltd. (Hadfield, UK), Sigma–Aldrich, and TCI Europe N.V. (Zwijndrecht, Belgium).

Balzulat A., Zhu W.F., Flauaus C., Hernandez‐Olmos V., Heering J., Sethumadhavan S., Dubiel M., Frank A., Menge A., Hebchen M., Metzner K., Lu R., Lukowski R., Ruth P., Knapp S., Müller S., Steinhilber D., Hänelt I., Stark H., Proschak E, & Schmidtko A. (2024). Discovery of a Small Molecule Activator of Slack (Kcnt1) Potassium Channels That Significantly Reduces Scratching in Mouse Models of Histamine‐Independent and Chronic Itch. Advanced Science, 11(15), 2307237.

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Calcium Signaling in Endothelial Cells

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Cell culture media and reagents were purchased from Life Technologies (Carlsbad, CA) or Lonza (Basel, Switzerland). Fetal calf serum was obtained from Biochrom (Berlin, Germany), and Quest Fluo‐8^™ AM and probenecid from AAT Bioquest. SLIGKV‐NH₂ was ordered from Bachem (Bubendorf, Switzerland). SLIGRL‐NH₂, TFLLR‐NH₂, trypsin, thrombin, ATP, and A23187, as well as EHop‐016, Gö6983, Y‐27632, PMA, and phosphatidylserine were purchased from Sigma‐Aldrich (St. Louis, MO). Cell staining reagents were purchased from Life Technologies. Plates (96 and 384 well) were obtained from Greiner Bio‐One. Teleocidin A2, a small molecule originally isolated from Streptomyces species, was a kind gift from IMD Natural Solutions, Dortmund, Germany. PAR1 inhibitor vorapaxar was purchased from Axon Medchem (Groningen, the Netherlands).

Stahn S., Thelen L., Albrecht I., Bitzer J., Henkel T, & Teusch N.E. (2016). Teleocidin A2 inhibits human proteinase‐activated receptor 2 signaling in tumor cells. Pharmacology Research & Perspectives, 4(4), e00230.

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Evaluation of TSLP Receptor Modulators

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Mouse TSLP was purchased from ProSpec (catalog #CYT-1135, East Brunswick, NJ, USA). GSK1016790A (catalog #G0798), HC067047 (catalog #616521), carvacrol (catalog #282197), 2-Aminoethoxydiphenylborane (2-APB, catalog #100065), SLIGRL-NH₂ (SLIGRL, catalog #S9317), FSLLRY-NH₂ (FSLLRY, catalog #SML0714), HC030031 (catalog #H4415), capsazepine (catalog #C191), baicalein (catalog #465119), dispase II (catalog #D4693), 4-nitrophenyl N-acetyl-β-D-glucosaminide (pNAG, catalog #N9376), and toluidine blue (catalog #89640) were purchased from Sigma-Aldrich (St, Louis, Missouri, USA).

Lee W.J, & Shim W.S. (2021). Cutaneous Neuroimmune Interactions of TSLP and TRPV4 Play Pivotal Roles in Dry Skin-Induced Pruritus. Frontiers in Immunology, 12, 772941.

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Mouse Keratinocytes and Melanoma Cell Culture

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Mouse keratinocytes (XB2 cell line), obtained from Dorothy Bennett and Elena Sviderskaya lab, were cultured in DMEM supplemented with 10% FBS, 2 mM l‐glutamine, 100 U/ml penicillin and 100 μg/ml streptomycin. Cells were maintained in a humidified incubator at 37°C and 10% CO₂. MNT‐1 human melanoma cells were maintained in DMEM containing 10% FBS, 2 mM l‐glutamine, non‐essential amino acids, sodium pyruvate, 100 U/ml penicillin and 100 μg/ml streptomycin. SLIGRL‐NH2, SFLLRN and synthetic melanin were purchased from Sigma (Darmstadt, Germany), and melanin from Sepia officinalis from Santa Cruz (Dallas, TX, USA).

Moreiras H., Bento‐Lopes L., Neto M.V., Escrevente C., Cabaço L.C., Hall M.J., Ramalho J.S., Seabra M.C, & Barral D.C. (2022). Melanocore uptake by keratinocytes occurs through phagocytosis and involves protease‐activated receptor‐2 internalization. Traffic (Copenhagen, Denmark), 23(6), 331-345.

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Culturing Mouse Keratinocytes and Human Melanoma

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Mouse keratinocytes (XB2 cell line), obtained from Dorothy Bennett and Elena Sviderskaya lab, were cultured in DMEM supplemented with 10% FBS, 2 mM L-Glutamine, 100 U/ml penicillin and 100 μg/ml streptomycin. Cells were maintained in a humidified incubator at 37°C and 10% CO2. MNT-1 human melanoma cells were maintained in DMEM containing 10% FBS, 2 mM L-Glutamine, non-essential amino acids, sodium pyruvate, 100 U/ml penicillin and 100 μg/ml streptomycin. SLIGRL-NH2, SFLLRN and synthetic melanin were purchased from Sigma (Darmstadt, Germany), and melanin from Sepia officinalis from Santa Cruz (Dallas, TX, USA).

Moreiras H., Neto M.V., Bento‐Lopes L., Escrevente C., Ramalho J.S., Seabra M.C., & Barral D.C. (2021). Melanocore uptake by keratinocytes occurs through phagocytosis and involves Protease-activated receptor-2 activation.

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