Cells (5× 105 cells/well) were seeded in a 6-well plate and treated with MLD. Annexin V and PI (4A BIOTECH, Beijing, China) were added to the cells and incubated for 15 min in the dark. Apoptotic cells were quantified using flow cytometry (Beckman FACSCalibur Flow Cytometer, Brea, CA, USA).
Annexin 5
Manufactured by 4A Biotech
Sourced in China
Annexin V is a protein that binds to phosphatidylserine, a phospholipid present on the surface of cells undergoing apoptosis (programmed cell death). It is commonly used in scientific research to detect and quantify apoptotic cells.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using annexin 5
1
Apoptosis Quantification by Flow Cytometry
2
Apoptosis Evaluation of GC Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
GC cells were treated with CHE (0, 5, 10, and 20 μmol/L) for 24 h, and adherent and floating cells were washed with PBS and harvested with trypsin. The washed cells were then collected by centrifugation and resuspended in staining buffer containing 5 μL annexin V and 10 μL PI (200 μg/mL) (4A Biotech, Beijing, China). Following incubation of the cells at room temperature for 5 min in the dark, the proportion of cells at different stages of apoptosis was quantified using flow cytometry. This experiment was performed in triplicate.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!