Log In Sign up
The largest database of trusted experimental protocols

Pf562271

Manufactured by Adipogen
Visit Supplier

PF562271 is a small-molecule compound developed by Adipogen. It is a selective and potent inhibitor of the PAK4 (p21-activated kinase 4) enzyme. PAK4 is a member of the PAK family of serine/threonine protein kinases and plays a role in various cellular processes. The function of PF562271 is to inhibit the activity of the PAK4 enzyme.

Automatically generated - may contain errors

Lab products found in correlation

Gefitinib, by Cayman Chemical (3 mentions) Zstk474, by Cell Signaling Technology (3 mentions) Latrunculin b, by Enzo Life Sciences (3 mentions) Ionomycin, by Thermo Fisher Scientific (2 mentions) Yohimbine, by Merck Group (2 mentions) Jasplakinolide, by Cayman Chemical (2 mentions) Dasatinib, by Cayman Chemical (2 mentions) Anisomycin, by Merck Group (2 mentions) Bapta am, by Selleck Chemicals (2 mentions) Ly2584702, by Selleck Chemicals (2 mentions) Gdc 0994, by Apexbio (2 mentions) Uk14304, by Merck Group (2 mentions) Y 27632, by Enzo Life Sciences (2 mentions) Rapamycin, by Cayman Chemical (1 mentions) Epidermal growth factor (egf), by Merck Group (1 mentions) Latrunculin a, by Adipogen (1 mentions) Ly294002, by Cayman Chemical (1 mentions)

3 protocols using pf562271

1

Chemical Compound Preparation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Stocks of 200 μM phorbol-12,13-dibutyrate (PDBu, EMD Millipore, #524390), 1 mg/mL anisomycin (Sigma-Aldrich, A9789), 10 mM UK14304 (Sigma-Aldrich, U104), 10 mM yohimbine (Sigma-Aldrich, Y3125), 1 mM gefitinib (Cayman, #13166), 1 mM ionomycin (Peprotech, #5608212), 2 mM jasplakinolide (Cayman, #11705), 25 mM latrunculin B (Enzo Life Sciences, BML-T110–0001), 10 mM PF562271 (AdipoGen, SYN-1064), 10 mM ZSTK474 (Cell Signaling, #13213), 10 mM dasatinib (Cayman, #11498), 10 mM GDC-0994 (APExBIO Technology, B5817), 1 mM LY2584702 (Selleck, S7698), 10 mM BAPTA-AM (Selleck, S7534) were prepared by dissolving the chemicals in DMSO; 10 mM Y27632 (Enzo Life Sciences, #ALX-270–333) in water. Stocks were diluted to the indicated final concentrations in the culture medium. The EGF stock solution was prepared by dissolving EGF (Sigma-Aldrich, E9644) in 10 mM acetic acid to a final concentration of 1 mg/ml. All drug stocks were stored at −20°C. 2-Deoxyglucose (2-DG, MilliporeSigma, #D8375) was dissolved in culture medium to 100 mM and used immediately.
Yang J.M., Chi W.Y., Liang J., Takayanagi S., Iglesias P.A, & Huang C.H. (2021). Deciphering cell signaling networks with massively multiplexed biosensor barcoding. Cell, 184(25), 6193-6206.e14.
+ Open protocol
+ Expand
2

Preparation of Pharmacological Reagents for Cell-Based Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
Stocks of 50 mM LY294002 (Cayman, #70920), 5 mM Latrunculin A (AdipoGen, #AGCN20027C100), 25 mM Latrunculin B (Enzo, #BML-T110-0001), 10 mM MEK inhibitor PD325901 (Calbiochem, #444966), 10 mM FAK inhibitor PF-573228 (Cayman, #14924), 10 mM FAK inhibitor PF-562271 (AdipoGen, SYN-1064), 10 mM ZSTK474 (Cell Signaling, #13213), 1 mM Gefitinib (Cayman, #13166), and 10 mM Rapamycin (Cayman, #13346) were prepared by dissolving the chemicals in DMSO. The stocks were diluted to the indicated final concentrations in culture medium. The EGF stock solution was prepared by dissolving EGF (Sigma, #E9644) in 10 mM acetic acid to a final concentration of 1 mg/ml. All drug stocks were stored at −20 °C.
Yang J.M., Bhattacharya S., West-Foyle H., Hung C.F., Wu T.C., Iglesias P.A, & Huang C.H. (2018). Integrating chemical and mechanical signals through dynamic coupling between cellular protrusions and pulsed ERK activation. Nature Communications, 9, 4673.
+ Open protocol
+ Expand
3

Chemical Compound Preparation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Stocks of 200 μM phorbol-12,13-dibutyrate (PDBu, EMD Millipore, #524390), 1 mg/mL anisomycin (Sigma-Aldrich, A9789), 10 mM UK14304 (Sigma-Aldrich, U104), 10 mM yohimbine (Sigma-Aldrich, Y3125), 1 mM gefitinib (Cayman, #13166), 1 mM ionomycin (Peprotech, #5608212), 2 mM jasplakinolide (Cayman, #11705), 25 mM latrunculin B (Enzo Life Sciences, BML-T110–0001), 10 mM PF562271 (AdipoGen, SYN-1064), 10 mM ZSTK474 (Cell Signaling, #13213), 10 mM dasatinib (Cayman, #11498), 10 mM GDC-0994 (APExBIO Technology, B5817), 1 mM LY2584702 (Selleck, S7698), 10 mM BAPTA-AM (Selleck, S7534) were prepared by dissolving the chemicals in DMSO; 10 mM Y27632 (Enzo Life Sciences, #ALX-270–333) in water. Stocks were diluted to the indicated final concentrations in the culture medium. The EGF stock solution was prepared by dissolving EGF (Sigma-Aldrich, E9644) in 10 mM acetic acid to a final concentration of 1 mg/ml. All drug stocks were stored at −20°C. 2-Deoxyglucose (2-DG, MilliporeSigma, #D8375) was dissolved in culture medium to 100 mM and used immediately.
Yang J.M., Chi W.Y., Liang J., Takayanagi S., Iglesias P.A, & Huang C.H. (2021). Deciphering cell signaling networks with massively multiplexed biosensor barcoding. Cell, 184(25), 6193-6206.e14.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!