Clopyralid

Manufactured by Merck Group

Clopyralid is a laboratory chemical used as an analytical standard or reagent. It is a pyridine carboxylic acid derivative. Clopyralid is used for various analytical and research applications, but a detailed description of its core function is not available while maintaining an unbiased and factual approach.

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4 protocols using clopyralid

Hapten Synthesis and Analytical Characterization

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The chemicals and reagents used for the synthesis of haptens were of analytical grade and were purchased from Sigma (St. Louis, MO) or Thermo Fisher Scientific (Rockford, IL). Bovine serum albumin (BSA), Ovalbumin (OVA), Thyroglobulin (Thy), 3,3’, 5,5’-tetramethylbenzidine (TMB), luminol and 4-iodophenol (PIP) were purchased from Sigma (St. Louis, MO). Goat anti-rabbit IgG-horseradish peroxidase was supplied by Abcam (Cambridge, MA). Standards (dicamba, 1, and its analogs, 5-hydroxydicamba, 2; 2,3,5-trichlorobenzoic acid, 3; 2,3,6-trichlorobenzoic acid, 4; clopyralid, 5; picloram, 6; chloramben, 7 and chlorfenac, 8) (Figure 1)) were purchased from Sigma (St. Louis, MO), Thermo Fisher Scientific (Rockford, IL) or Chem Service, Inc. (West Chester, PA). OriginPro 8.1 (OriginLab, Northampton, MA) was used for processing of the analytical data.

Huo J., Barnych B., Li Z., Wan D., Li D., Vasylieva N., Knezevic S.Z., Osipitan O.A., Scott J.E., Zhang J, & Hammock B.D. (2019). Hapten Synthesis, Antibody Development, and a Highly Sensitive Indirect Competitive Chemiluminescent Enzyme Immunoassay for Detection of Dicamba. Journal of agricultural and food chemistry, 67(20), 5711-5719.

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Synthesis of Metal Complexes and Purification

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Clopyralid (99%), titanium (IV) butoxide (97%), ruthenium (II) chloride (RuCl3.xH2O, 99.9%) and zinc acetate (99%) were purchased from Sigma-Aldrich. Potassium nitrate and anhydrous sodium sulfate were from Panreac. Methanol and HPLC grade formic acid were obtained Sigma-Aldrich. All aqueous solutions were prepared with ultrapure water from a Millipore Mili-Q Gradient system (resistivity 18.2 MΩ cm).

Goulart L.A., Santos G.O.S., Eguiluz K.I.B., Salazar-Banda G.R., Lanza M.R.V., Saez C, & Rodrigo M.A. (2021). Towards a higher photostability of ZnO photo-electrocatalysts in the degradation of organics by using MMO substrates. Chemosphere, 271.

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Analytical-Grade Reagents for HPLC

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Clopyralid was of analytical grade (99%), purchased from Sigma Aldrich. Sodium chloride and anhydrous sodium sulfate used as supporting electrolytes were of analytical grade and purchased from Panreac. Methanol and formic acid used for the mobile phase of HPLC were purchased from Sigma-Aldrich. All aqueous solutions were prepared using high-purity water (Millipore Milli-Q system, resistivity > 18 MΩ). All reactants were used as received.

Santos G.O.S., Eguiluz K.I.B., Salazar-Banda G.R., Saez C, & Rodrigo M.A. (2020). Biodegradability improvement of clopyralid wastes through electrolysis using different diamond anodes. Environmental research, 188.

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Clopyralid-Based Assay Development

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Clopyralid (CAS Number 1702-17-6), sodium hydroxide, ethidium bromide, di-sodium salt of ethylene diamine tetra acetic acid (EDTA), trizma base, sodium chloride, hydrochloric acid, disodium hydrogen phosphate, MMS (CAS Number 67-27-3), low melting point agarose (LMPA), potassium phosphate monobasic, magnesium chloride hexahydrate, potassium chloride, triton X-100, normal melting point agarose (NMPA), basic fuchsin, and trizma hydrochloride were purchased from Sigma-Aldrich.

Amaç E., & Liman R. (2021). Cytotoxic and Genotoxic Effects of Clopyralid Herbicide on Allium Cepa Roots.

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