Anti cd11c alexa 700

Manufactured by BioLegend

Anti-CD11c Alexa 700 is a fluorescently labeled antibody that binds to the CD11c cell surface marker, which is expressed on dendritic cells and macrophages. This product can be used for the identification and analysis of these cell types in flow cytometry applications.

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2 protocols using anti cd11c alexa 700

Multiparameter Flow Cytometry Protocol

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The following monoclonal Abs were used in flow cytometry: anti-CD45 (30–11, APC-Cy7, PE), anti-CD8 (Ly-2, V450), anti-CD4 (RM4-5, V500) anti-CD44 (IM7, Alexa 700) anti-mouse OVA257-264 (SIINFEKL) peptide bound to H-2Kb (PE), anti-CD326 (EPCAM) (9C4; FITC), anti-CD24 (30-F1, PE-Cy7), anti-CD69 (H1.2F3, PerCP/Cy5.5), anti-CD61 (Ebioscience, 2C9.G3; FITC) anti-PDGRFα CD140α (Abcam, 16A1; PE); anti-Ly6C PE, anti-Ly6G BV785, anti-Foxp3 Alexa 488, anti-F4/80 Pe-Cy7, anti-CD206 BV650, anti-CD11b Percp Cy5, anti-CD86 BV605, anti-CD80 APC-Cy7, anti-IFNγBV650, anti-GrzB Alexa647, anti-TNFα BV605, anti-NKp46 Alexa 488, anti-CD107a Pe-Cy7, anti-CD29 Percp Cy5, anti-PD-1 PE, anti-Tim3 APC, anti-Lag3 Percp Cy5 and anti-CD11c Alexa 700 (all from Biolegend). The flow cytometry analyses were performed using a BD Fortessa Flow Cytometer (BD Biosciences) and analysed using either BD FACS Diva software V.5.0.1 (BD) or FlowJo (Tree Star). For the in vivo studies, the following endotoxin-free Abs were used: anti-CD8 (BioXcell; 2.43), anti-βigh3 18B3,51 (link) anti-PD-1 and control polyclonal mouse Ig (BioXcell).

Goehrig D., Nigri J., Samain R., Wu Z., Cappello P., Gabiane G., Zhang X., Zhao Y., Kim I.S., Chanal M., Curto R., Hervieu V., de La Fouchardière C., Novelli F., Milani P., Tomasini R., Bousquet C., Bertolino P, & Hennino A. (2018). Stromal protein βig-h3 reprogrammes tumour microenvironment in pancreatic cancer. Gut, 68(4), 693-707.

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Comprehensive Multiparameter Flow Cytometry

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Supernatants were removed and cell pellets were resuspended in 200 μL of staining buffer containing 1 μL each of anti-CD38-APC (BioLegend, clone HIT2), anti-CD13-PE-Cy7 (BioLegend, clone WM15), anti-CD21-PE-Dazzle (BioLegend, clone Bu32), anti-CD19-APC-efluor-780 (Invitrogen, clone HIB19), anti-IgD-PerCP-Cy5.5 (Biolegend, clone IA6–2), anti-IgM-FITC (Biolegend, clone MHM-88), anti-CD27-BV510 (Biolegend, clone M-T271), anti-CD11c-Alexa700 (BioLegend, clone Bu15). Staining buffer also contained Delta-S1-PE and S1-BV421 tetramers.

Quirk G.E., Schoenle M.V., Peyton K.L., Uhrlaub J.L., Lau B., Burgess J.L., Ellingson K., Beitel S., Romine J., Lutrick K., Fowlkes A., Britton A., Tyner H.L., Caban-Martinez A.J., Naleway A., Gaglani M., Yoon S., Edwards L., Olsho L., Dake M., LaFleur B.J., Nikolich J.Ž., Sprissler R., Worobey M, & Bhattacharya D. (2023). Determinants of de novo B cell responses to drifted epitopes in post-vaccination SARS-CoV-2 infections. medRxiv.

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