H&E staining was performed using H&E staining kit (C0105, Beyotime, China) according to the manufacture's instructions. Immunohistochemistry (IHC) stainings of HER2 was performed using anti-HER2/neu antibody (4B5, Roche, Basel, Switzerland) according to a previous report31 (link). H&E staining and IHC staining were reviewed and scored according to the criteria reported previously32 (link) by two independent pathologists who were blinded to this study.
Anti her2 neu antibody 4b5
Manufactured by Roche
Sourced in Switzerland
The Anti-HER2/neu antibody (4B5) is a laboratory reagent used for the detection and quantification of the HER2/neu protein in biological samples. It is a monoclonal antibody that specifically binds to the HER2/neu receptor, which is overexpressed in certain types of cancer cells. The antibody can be used in various immunoassay techniques, such as immunohistochemistry (IHC) and enzyme-linked immunosorbent assay (ELISA), to measure the expression levels of HER2/neu in tissue or fluid samples.
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Lab products found in correlation
2 protocols using anti her2 neu antibody 4b5
1
Histological Assessment of HER2 Expression
2
HER2 Expression and Amplification Testing
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HER2 protein expression was tested using anti‐HER2/neu antibody (4B5; Roche, Basel, Switzerland) by IHC staining according to a previous report.13 HER2 amplification was determined using Ventana HER2 dual‐color ISH assay (DISH; Ventana Medical Systems, Inc., Tucson, AZ, USA). Amplification of HER2 by DISH was defined as a ratio HER2/CEP17 ≥2.2, and two independent specialists who were blinded to the study read the result.
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