Log In Sign up
The largest database of trusted experimental protocols

Mouse anti g9a clonal

Manufactured by R&D Systems
Visit Supplier

Mouse anti-G9a (clonal) is a monoclonal antibody that recognizes the G9a (also known as EHMT2 or KMT1C) protein. G9a is a histone methyltransferase that catalyzes the mono- and di-methylation of histone H3 at lysine 9 (H3K9me1 and H3K9me2).

Automatically generated - may contain errors

Lab products found in correlation

Sybr green pcr kit, by Thermo Fisher Scientific (2 mentions) Magna chip kit, by Merck Group (2 mentions) Mouse monoclonal anti flag m2 f 3165, by Merck Group (2 mentions) Rabbit anti g9a clonal c6h3, by Cell Signaling Technology (2 mentions) Mouse anti h3k9me2 ab1220, by Abcam (2 mentions)

Close spelling variants of this product

Anti-G9a

2 protocols using mouse anti g9a clonal

1

Chromatin Immunoprecipitation (ChIP) Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
ChIP analysis was performed using Magna ChIP kits (Millipore) according to the manufacturer’s instruction. The following antibodies were used in ChIP: mouse monoclonal anti-FLAG (M2) (F-3165, Sigma-Aldrich) (for FLAG-cyclin D1), mouse anti-G9a (clonal A8620A) (PP-A8620A-00) (R&D System), rabbit anti-G9a (clonal C6H3) (Cell signaling), and mouse anti-H3K9me2 (ab1220) (Abcam Inc.). For a negative control, mouse IgG was from the Magna ChIP kits (Millipore). SYBR Green PCR kit (Invitrogen) was used in the ChIP-real time PCR (ChIP-qPCR). The primers used in ChIP-qPCR are shown in the Supplemental Table 1.
Li Z., Jiao X., Di Sante G., Ertel A., Casimiro M.C., Wang M., Katiyar S., Ju X., Klopfenstein D.V., Tozeren A., Dampier W., Chepelev I., Jeltsch A, & Pestell R.G. (2019). Cyclin D1 integrates G9a-mediated histone methylation. Oncogene, 38(22), 4232-4249.
+ Open protocol
+ Expand
2

Chromatin Immunoprecipitation (ChIP) Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
ChIP analysis was performed using Magna ChIP kits (Millipore) according to the manufacturer’s instruction. The following antibodies were used in ChIP: mouse monoclonal anti-FLAG (M2) (F-3165, Sigma-Aldrich) (for FLAG-cyclin D1), mouse anti-G9a (clonal A8620A) (PP-A8620A-00) (R&D System), rabbit anti-G9a (clonal C6H3) (Cell signaling), and mouse anti-H3K9me2 (ab1220) (Abcam Inc.). For a negative control, mouse IgG was from the Magna ChIP kits (Millipore). SYBR Green PCR kit (Invitrogen) was used in the ChIP-real time PCR (ChIP-qPCR). The primers used in ChIP-qPCR are shown in the Supplemental Table 1.
Li Z., Jiao X., Di Sante G., Ertel A., Casimiro M.C., Wang M., Katiyar S., Ju X., Klopfenstein D.V., Tozeren A., Dampier W., Chepelev I., Jeltsch A, & Pestell R.G. (2019). Cyclin D1 integrates G9a-mediated histone methylation. Oncogene, 38(22), 4232-4249.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!