Cells were stained with a viability dye (Live/Dead fixable near-IR dead cell stain kit, Invitrogen) and fluorochrome-labelled antibodies for 30 minutes at 4°C. The following antibodies were used: CD3-eFluor450 (OKT3, eBioscience), CD8-VioGreen (BW135/80, Miltenyi), γδTCR-FITC (5A6.E9, Invitrogen), CD161-PE and CD161-APC (191B8, Miltenyi), Vδ2-PerCP-Cy5.5 (B6, BioLegend), CD56-PE-Cy7 (HCD56, BioLegend), Vα7.2-APC and Vα7.2-PerCP-Cy5.5 (3C10, BioLegend), CCR7-FITC (G043H7), CD62L-PE (DREG-56, BioLegend). Data was acquired by a MACSQuant Analyzer 10 (Miltenyi).
Cd161 pe and cd161 apc
Manufactured by Miltenyi Biotec
CD161-PE and CD161-APC are fluorescently-labeled antibodies used for the identification and analysis of CD161-positive cells in flow cytometry applications. CD161 is a type II membrane protein expressed on a subset of T cells and natural killer (NK) cells. These antibodies can be used to detect and quantify CD161-expressing cells in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Vδ2 percp cy5.5 b6, by BioLegend (2 mentions)
Cd62l pe dreg 56, by BioLegend (2 mentions)
Cd3 efluor450 okt3, by Thermo Fisher Scientific (2 mentions)
Cd56 pe cy7 hcd56, by BioLegend (2 mentions)
Ccr7 fitc g043h7, by BioLegend (2 mentions)
Live dead fixable near ir dead cell stain kit, by Thermo Fisher Scientific (2 mentions)
Macsquant analyzer 10, by Miltenyi Biotec (2 mentions)
Cd8 viogreen, by Miltenyi Biotec (1 mentions)
2 protocols using cd161 pe and cd161 apc
1
Multiparameter Flow Cytometry Staining
2
Immunophenotyping of T Cell Subsets
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Cells were stained with a viability dye (LIVE/DEAD Fixable Near-IR Dead Cell Stain Kit; Invitrogen) and fluorochrome-labeled Abs for 30 min at 4°C. The following Abs were used: CD3–eFluor 450 (OKT3; eBioscience), CD8-VioGreen (BW135/80; Miltenyi Biotec), γδTCR-FITC (5A6.E9; Invitrogen), CD161-PE and CD161-APC (191B8; Miltenyi Biotec), Vδ2-PerCP-Cy5.5 (B6; BioLegend), CD56–PE-Cy7 (HCD56; BioLegend), Vα7.2-APC and Vα7.2-PerCP-Cy5.5 (3C10; BioLegend), CCR7-FITC (G043H7), and CD62L-PE (DREG-56; BioLegend). Data were acquired by a MACSQuant Analyzer 10 (Miltenyi Biotec).
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