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1420 arvo x

Manufactured by PerkinElmer
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The 1420 ARVO X is a multimode microplate reader designed for a variety of quantitative and qualitative assays. It can detect a wide range of detection technologies, including absorbance, fluorescence, and luminescence.

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Lab products found in correlation

12 well cell culture insert, by Corning (2 mentions) Workout plus software, by PerkinElmer (2 mentions)

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ARVO X (7 citations)

2 protocols using 1420 arvo x

1

In Vitro Epithelial Permeability Assay

Cited 1 time
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FITC-tracers were prepared as previously described10 (link). To assess barrier function, in vitro epithelial permeability assays were performed using 12-well cell culture inserts (353180; Corning), as previously described10 (link). Fluorescence intensity was determined using a 1420 ARVO X (PerkinElmer). Data were analyzed using the WorkOut Plus software package (PerkinElmer).
Yamaga S., Tanigaki K., Nakamura E., Sasaki N., Kato Y., Kuboniwa M., Matsusaki M., Amano A, & Takeuchi H. (2023). Cigarette smoke extract impairs gingival epithelial barrier function. Scientific Reports, 13, 9228.
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2

Cell Barrier Permeability Assay

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The in vitro epithelial permeability assay to assess barrier function was performed with 12-well cell culture inserts (353180; Corning). When IHGE cells in the upper compartment reached 100% confluence, 20 μL of 0.1 mg mL-1 Lucifer yellow, FITC-dextran, FITC-LPS, or FITC-PGN was added to the upper compartment of the insert. After a 30-min incubation, the medium was collected from the lower compartment, and the fluorescence intensity was measured using 1420 ARVO X (PerkinElmer). Data were acquired using the WorkOut Plus software (PerkinElmer).
Takeuchi H., Sasaki N., Yamaga S., Kuboniwa M., Matsusaki M, & Amano A. (2019). Porphyromonas gingivalis induces penetration of lipopolysaccharide and peptidoglycan through the gingival epithelium via degradation of junctional adhesion molecule 1. PLoS Pathogens, 15(11), e1008124.
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