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Il 25

Manufactured by Merck Group
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IL-25 is a laboratory equipment product from Merck Group. It is used to measure and quantify the levels of interleukin-25 (IL-25), a cytokine involved in immune system regulation and inflammatory responses. The core function of IL-25 is to facilitate the detection and analysis of this specific protein in biological samples.

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Lab products found in correlation

Il 25, by R&D Systems (1 mentions) Ly294002, by Merck Group (1 mentions) Tgf β1, by R&D Systems (1 mentions) 4 pba, by Merck Group (1 mentions) Thapsigargin, by Merck Group (1 mentions) Bioscript, by Meridian Bioscience (1 mentions) Trizol rna extraction, by Thermo Fisher Scientific (1 mentions) Sybr green, by Thermo Fisher Scientific (1 mentions) Rnalater, by Thermo Fisher Scientific (1 mentions)

3 protocols using il 25

1

Cardiac Fibroblast Response to Cytokines

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CFs were cultured as described previously [32 (link)–35 ]. CFs were then divided into four groups according to cell treatment, namely: (1) control group, (1%FBS-DMEM); (2) TGF-β1 treatment group (1%FBS-DMEM + TGF-β1[10 ng/ml, R&D Systems]); (3) IL-25 treatment group(1%FBS-DMEM + IL-25 [10 ng/ml, R&D Systems]); and (4) IL-25 + PI3K-antagonist group (1%FBS-DMEM + IL-25 [10 ng/ml] + LY294002 [15 μM, Sigma-Aldrich)]). Cells were then cultured for another 24 h and then harvested for further analyses, including real-time PCR, western blotting, ELISA, and cell proliferation and migration analyses.
Yao X., Chen Q., Wang X., Liu X, & Zhang L. (2023). IL-25 induces airway remodeling in asthma by orchestrating the phenotypic changes of epithelial cell and fibrocyte. Respiratory Research, 24, 212.
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2

Apoptosis Evaluation in 16HBE Cells

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16HBE cells were seeded in 6 well plates and treated with treated with IL-25 (100 ng/ml), thapsigargin (Tg, 0.5uM) and 4-PBA (5 mM; Sigma, St Louis, USA) respectively in a serum-free culture medium. After treatment 2 hours with 4-PBA, the medium containing IL-25 (100 ng/ml) was added to co-incubate cells. Following treatment, the cells were harvested by 0.2% trypsin. 106 cells were suspended in 100ul binding buffer with 5ul Annexin V-FITC and 5ul PI staining solution for 10 min at room temperature. The samples were set to analysed by a FACS array. Data were analyzed by software flowjo (FlowJo flow cytometry analysis software, Tree Star, USA).
Yuan X., Wang J., Li Y., He X., Niu B., Wu D., lan N., Wang X., Zhang Y., Dai X., Wang X., Liu Z, & Li G. (2018). Allergy immunotherapy restores airway epithelial barrier dysfunction through suppressing IL-25 -induced endoplasmic reticulum stress in asthma. Scientific Reports, 8, 7950.
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3

Quantifying Esophageal Gene Expression

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Mouse oesophagi were immersed in RNAlater ® (Ambion, Life Technologies Australia, Mulgrave, Australia) before being frozen at −80°C. Total RNA was then isolated using TRIzol® RNA extraction (Invitrogen, Life Technologies Australia, Mulgrave, Australia) and reverse transcribed to cDNA using BioScript (Bioline, Alexandria, Australia).
Gene expression within the oesophagus was determined using RT-qPCR (Eppendorf Realplex, Hamburg, Germany) and SYBR Green (Invitrogen, Life Technologies Australia, Mulgrave, Australia). Primers specific for murine MID1 (Forward: 5-CACTCGCTGAAGGAAAATGACCA-3, Reverse: 5-AATCCAAGGCAAAAGTGTCAAA CG-3), CCL11 (F: 5-TTCTATTCCTGCTGCTCACGG-3, R: 5-AGGGTGCATCTGTTGT TGGTG-3), TGF-β (F: 5-TGTGGAACTCTACCAGAAATATAGC-3, R: 5-GAAAGCCCT GTATTCCGTCTC-3), TSLP (F:5-AGGCTACCCTGAAACTGAG-3, R:5-GGAGATTGCA TGAAGGAATACC-3) CCL20 (F:5-CGACTGTTGCCTCTCGTACA-3, R: 5-AGGAGGTTCACAGCCCTTTT-3) IL-25 (F: 5-ATGTACCAGGCTGTTGCATTCTTG-3) (R: 5-CTAAGCCATGACCCGGGGCC-3) (Sigma-Aldrich, Castle Hill, Australia) and CCL24 (Biomol, VMPS-907, Enzo Life Sciences, Farmingdale, NY) were used to quantify mRNA copy numbers as described previously [38 (link)]. Murine β-actin was used as a housekeeper gene and gene expression was determined as mRNA copies of the gene of interest per copy of β-actin (F:5-GACGGCCAGGTCATCACTATTG-3, R:5-AGGAA GGCTGGAAAAGAGCC-3).
Collison A., Sokulsky L.A., Sherrill J.D., Nightingale S., M.C.l.i.n.E.p.i.d., Hatchwell L., Talley N.J., Walker M.M., Rothenberg M.E, & Mattes J. (2015). TRAIL regulates MID1, TSLP, inflammation and remodelling in experimental eosinophilic oesophagitis. The Journal of allergy and clinical immunology, 136(4), 971-982.
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