Mouse experiments were conducted in accordance with Institutional Animal Care and Committee (IACUC) protocols and Animal Resource Center (ARC) at Case Western Reserve University. Cre-ERT2 and Pten knockout mice were obtained from Jackson Laboratory. Sprr2f-Cre mice were provided by Dr. Leone. Cyclin D1Flox/WT mice were generated in Cyagen Biosciences Inc. Tamoxifen (Sigma) was administered orally to Cyclin D1Flox/WT Cre-ERT2 once a day for 5 days at a dose of 200mg/kg of body weight to generate Cyclin D1TA/WT mice. 1–2 mg of BrdU was administered by intraperitoneal injection two hours prior to analysis. The genotypes and DNA recombination were verified by genomic PCR. PCR primers are as follows: cyclin D1Flox/WT F1: 5’-GTGGAAGGCTTCCAGGTGTGAC-3’, R1: 5’-CCTAGTATGGACCCACAGACACCC-3’, F3: 5’-GCCCCATTCCTACATATCTCA-3’ and R3: 5’-TGGATCCACCTAATAACTTCGTA-3’.
Cyclin d1flox wt mice
Manufactured by Cyagen
The Cyclin D1Flox/WT mice are a genetically engineered mouse model. The model contains a floxed allele of the Cyclin D1 gene, which allows for conditional manipulation of Cyclin D1 expression in various cell types and tissues.
Automatically generated - may contain errors
2 protocols using cyclin d1flox wt mice
1
Conditional Cyclin D1 Deletion Mice
2
Conditional Cyclin D1 Deletion Mice
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Mouse experiments were conducted in accordance with Institutional Animal Care and Committee (IACUC) protocols and Animal Resource Center (ARC) at Case Western Reserve University. Cre-ERT2 and Pten knockout mice were obtained from Jackson Laboratory. Sprr2f-Cre mice were provided by Dr. Leone. Cyclin D1Flox/WT mice were generated in Cyagen Biosciences Inc. Tamoxifen (Sigma) was administered orally to Cyclin D1Flox/WT Cre-ERT2 once a day for 5 days at a dose of 200mg/kg of body weight to generate Cyclin D1TA/WT mice. 1–2 mg of BrdU was administered by intraperitoneal injection two hours prior to analysis. The genotypes and DNA recombination were verified by genomic PCR. PCR primers are as follows: cyclin D1Flox/WT F1: 5’-GTGGAAGGCTTCCAGGTGTGAC-3’, R1: 5’-CCTAGTATGGACCCACAGACACCC-3’, F3: 5’-GCCCCATTCCTACATATCTCA-3’ and R3: 5’-TGGATCCACCTAATAACTTCGTA-3’.
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