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2 protocols using water soluble free cholesterol

1

Lipidomic Profiling of Metabolic Disorders

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Antibody against LDLR (Cat # ab-30532),  MCT-1 (Cat # ab-90582), MCT-3 (Cat # ab-60333) and SR-B1 (Cat # 217318) were purchased from Abcam (Cambridge, UK); antibodies against FASN (Cat # sc-55580), MCT-4 (Cat # sc-50329), MCT-2 (Cat # sc-166925), LDHA (Cat # sc-137244), LXRα/β (Cat # sc-377260), ACAT-1 (Cat # sc-517387), ACAT-2 (Cat # sc-293307), phospho-ERK (Tyr 204) (Cat # sc-7383), ERK (Cat # sc-154), Cyclin A (Cat # sc-751), CDK 2 (Cat # sc-163), Cyclin E (Cat # sc-198), β-tubulin (Cat # sc-9104), GAPDH (Cat # sc-20357), β-actin (Cat # sc-1615), and HRP conjugated secondary antibodies: anti-rabbit (Cat # sc-2030), anti-mouse (Cat # sc-2031), anti-goat (Cat # sc-2033) were purchased from Santa Cruz Biotechnology (CA, USA). Human LDL cholesterol (Cat # 360–10) and human HDL cholesterol (Cat # 361–10) were purchased from Lee Biosolution (MO, USA); Water soluble-free cholesterol and Dextran sodium sulfate (DSS) (Cat # 160110) were purchased from MP Biomedicals (CA, USA). Azoxymethane (AOM) (Cat # A5486), 2-Deoxy-D-glucose (2-DG) (Cat # D8375) and Oxamate (Cat # O2751) were purchased from Sigma Aldrich (MO, USA).
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2

Lipidomic Profiling of Metabolic Disorders

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibody against LDLR (Cat # ab-30532),  MCT-1 (Cat # ab-90582), MCT-3 (Cat # ab-60333) and SR-B1 (Cat # 217318) were purchased from Abcam (Cambridge, UK); antibodies against FASN (Cat # sc-55580), MCT-4 (Cat # sc-50329), MCT-2 (Cat # sc-166925), LDHA (Cat # sc-137244), LXRα/β (Cat # sc-377260), ACAT-1 (Cat # sc-517387), ACAT-2 (Cat # sc-293307), phospho-ERK (Tyr 204) (Cat # sc-7383), ERK (Cat # sc-154), Cyclin A (Cat # sc-751), CDK 2 (Cat # sc-163), Cyclin E (Cat # sc-198), β-tubulin (Cat # sc-9104), GAPDH (Cat # sc-20357), β-actin (Cat # sc-1615), and HRP conjugated secondary antibodies: anti-rabbit (Cat # sc-2030), anti-mouse (Cat # sc-2031), anti-goat (Cat # sc-2033) were purchased from Santa Cruz Biotechnology (CA, USA). Human LDL cholesterol (Cat # 360–10) and human HDL cholesterol (Cat # 361–10) were purchased from Lee Biosolution (MO, USA); Water soluble-free cholesterol and Dextran sodium sulfate (DSS) (Cat # 160110) were purchased from MP Biomedicals (CA, USA). Azoxymethane (AOM) (Cat # A5486), 2-Deoxy-D-glucose (2-DG) (Cat # D8375) and Oxamate (Cat # O2751) were purchased from Sigma Aldrich (MO, USA).
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