Ml120b

Manufactured by Merck Group

Sourced in United States

The ML120B is a laboratory centrifuge designed for general-purpose applications. It features a fixed-angle rotor and can accommodate a variety of sample tubes. The centrifuge operates at a maximum speed of 6,000 RPM and can generate a maximum relative centrifugal force (RCF) of 4,650 x g. The unit is suitable for common laboratory tasks such as sample preparation, cell separation, and DNA/RNA purification.

Automatically generated - may contain errors

Lab products found in correlation

Goat anti mouse igg fitc, by Santa Cruz Biotechnology (1 mentions) Anti phospho nf κb p65 ser536, by Cell Signaling Technology (1 mentions) Anti phospho iκbα ser32, by Cell Signaling Technology (1 mentions) Goat anti rabbit igg hrp, by Abcam (1 mentions) Goat anti mouse igg hrp, by Abcam (1 mentions) Goat anti rabbit igg pe, by Santa Cruz Biotechnology (1 mentions) Anti foxp3, by Novus Biologicals (1 mentions) Anti nf κb p65, by Cell Signaling Technology (1 mentions) Anti iκbα, by Cell Signaling Technology (1 mentions) Anti gapdh, by Abcam (1 mentions) Tumor necrosis factor (tnf), by R&D Systems (1 mentions) Recombinant human il1b, by R&D Systems (1 mentions) Ps 1145, by Merck Group (1 mentions) Ifn γ, by R&D Systems (1 mentions) Mg 262, by Apexbio (1 mentions) Dexamethasone (dex), by Steraloids (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Cycloheximide (chx), by Merck Group (1 mentions) Mg132, by Merck Group (1 mentions) Budesonide, by AstraZeneca (1 mentions)

4 protocols using ml120b

Investigating CCL28-Mediated Immune Regulation

Check if the same lab product or an alternative is used in the 5 most similar protocols

The antibodies and reagents were obtained from the following sources: anti-CCL28 (Santa Cruz, sc-376654), anti-GAPDH (Abcam, ab9485), anti-phospho-NF-κB p65 (Ser536) (Cell Signaling, 3033), anti-NF-κB p65 (Cell Signaling, 8242), anti-phospho-IκBα (Ser32) (Cell Signaling, 2859), anti-IκBα (Cell Signaling, 4812), anti-FoxP3 (Novus, NB100-39002), anti-CCR10 (Invitrogen, PA1-21617), ML120B (Sigma, SML1174). Goat anti-rabbit IgG-HRP (Abcam, ab6721), goat anti-mouse IgG-HRP (Abcam, ab6789), goat anti-rabbit IgG-PE (Santa Cruz, sc-3739), goat anti-mouse IgG-FITC (Santa Cruz, sc-2010), goat anti-mouse peroxidase-conjugated IgG (Millipore, AP124P). Neutralizing antibodies against mouse IL-1β (AF401), TNF-α (AF410), CCL28 (MAB533), CCR10 (MAB2815), CCR3 (MAB1551), CD25 (AF2438) and IgG isotype control (43414), and recombinant mouse CCL28 (533-VI) were purchased from R&D Systems.

Wang P., Qi X., Xu G., Liu J., Guo J., Li X., Ma X, & Sun H. (2019). CCL28 promotes locomotor recovery after spinal cord injury via recruiting regulatory T cells. Aging (Albany NY), 11(18), 7402-7415.

+ Open protocol

+ Expand

Cytokine and Pharmacological Treatments

Check if the same lab product or an alternative is used in the 5 most similar protocols

Recombinant human IL1B, TNF and IFNG (R&D Systems, Minneapolis, MN) were dissolved in PBS containing 0.1% bovine serum albumin (Sigma-Aldrich, St. Louis, MO). Budesonide (AstraZeneca, Mölndal, Sweden), dexamethasone (Steraloids, Newport, RI), MG-132 (Millipore Sigma, St. Louis, MO), MG-262 (APExBIO, Houston, TX), PR-171 (Adooq BioScience, Irvine, CA), E-64 (APExBIO, Houston, TX), PS-1145, ML-120B (both Sigma-Aldrich) and Org34517 (gift from Chiesi Farmaceutici, Parma, Italy) were dissolved in dimethyl sulfoxide as stocks of 10 mM. Cycloheximide (Sigma-Aldrich) was dissolved in water at 10 mg/ml.

Thorne A., Bansal A., Necker-Brown A., Mostafa M.M., Gao A., Georgescu A., Kooi C., Leigh R, & Newton R. (2023). Differential regulation of BIRC2 and BIRC3 expression by inflammatory cytokines and glucocorticoids in pulmonary epithelial cells. PLOS ONE, 18(6), e0286783.

+ Open protocol

+ Expand

Investigating MAPK Signaling Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols

All reagents were purchased from commercial sources; LPS (InvivoGen, San Diego, CA, USA), SB203580 (Wako, Osaka, Japan), SP600125 (Wako), U0126 (Wako), 5z-7 (Santa Cruz, Dallas, TX, USA), ML120B (Sigma, St. Louis, MO, USA), TPL2 kinase inhibitor (Cayman Chemical, Ann Arbor, MI, USA), MG132 (Santa Cruz), poly(I:C) (InvivoGen), and Flagellin (InvivoGen). Antibodies against the following proteins were used in this study: p38, phospho-p38, JNK, phospho-JNK, ERK, phospho-ERK, p65, MKP1, MKP5, K48-linked polyubiquitin (Cell Signaling, Danvers, MA, USA), Myc-562 (MBL, Tokyo, Japan), FLAG-1E6 (Sigma), FLAG-M2 (Wako), HA (Roche, Basel, Switzerland), and PARP1, Myc-9E10, and β-actin (Santa Cruz). Mouse IgG, rat IgG, and rabbit IgG (Cell Signaling) were also used in this study. Anti-LINCR was produced by Cosmobio (Tokyo, Japan) using a part of the mouse-LINCR sequence (CDDQRSTARRRSTFHDGIV) as the antigen.

Yokosawa T., Miyagawa S., Suzuki W., Nada Y., Hirata Y., Noguchi T, & Matsuzawa A. (2024). The E3 Ubiquitin Protein Ligase LINCR Amplifies the TLR-Mediated Signals through Direct Degradation of MKP1. Cells, 13(8), 687.

+ Open protocol

+ Expand

Protein Expression and Phosphorylation Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The antibodies and reagents were purchased from the following sources: NCOA3 (F-2) (Santa Cruz, sc5305), GAPDH (0411) (Santa Cruz, sc47724), COL2A1 (M2139) (Santa Cruz, sc52658), Aggrecan (6-B-4) (abcam, ab3778), COL1A1 (3G3) (Santa Cruz, sc293182), p65 (abcam, ab16502), phospho-p65 (S536) (abcam, ab28856), IκBα (C-21) (Santa Cruz, sc371), phospho-IκBα (Ser32/36) (Cell Signaling, #9246), Goat anti-rabbit IgG-HRP (Santa Cruz, sc-2004), Goat anti-mouse IgG-HRP (Santa Cruz, sc-2302). 5-Azacytidine (Sigma, A2385), Bufalin (Sigma, B0261), BrdU (5-Bromo-2'-deoxyuridine) (Millipore, #19-160), ML120B (Sigma, SML1174), recombinant IL-1β (R&D System, NP-000567).

Zhang B., Li Z., Wang W., Guo J., Kang S., Liu S., Li H., Wang D, & Qi X. (2018). NCOA3 Loss Disrupts Molecular Signature of Chondrocytes and Promotes Posttraumatic Osteoarthritis Progression. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 49(6).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!