Western blotting analysis was conducted as previously reported [19 (link)]. The primary antibodies used were anti-HSC70 antibody (MAB2191; Abnova, Taipei, Taiwan), anti-CD63 antibody (sc-5725; Santa Cruz Biotechnology, Texas, USA), and anti-CYCS antibody (sc-13156), and the secondary antibodies used were horseradish peroxidase conjugated anti-rat IgG antibodies (81–9520; Invitrogen) and anti-mouse IgG antibodies (115-035-044; Jackson ImmunoResearch, West Grove, PA, USA). Signals were visualized using an Immunostar LD Kit (Wako, Tokyo, Japan) and the C-DiGit Blot Scanner and Image Studio for C-DiGit (LI-COR, Lincoln, NE, USA) according to the manufacturer’s protocols.
C digit blot scanner and image studio for c digit
Manufactured by LI COR
Sourced in Japan
The C-DiGit Blot Scanner is a compact and high-performance imaging system designed for Western blot analysis. It uses a CCD camera to capture digital images of chemiluminescent or fluorescent blots. The accompanying Image Studio for C-DiGit software provides tools for image acquisition, analysis, and quantification.
Automatically generated - may contain errors
2 protocols using c digit blot scanner and image studio for c digit
1
Western Blotting Analysis of Cellular Proteins
2
Western Blot Analysis of EV Markers
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The EV‐enriched fraction and MGCs were resuspended in Laemmli buffer (Laemmli, 1970 ) and immediately boiled for 5 min. Western blot analysis was conducted as reported previously (Matsuno et al., 2019 ). The primary antibodies used were anti‐HSC70 rat antibody (MAB2191; Abnova), anti‐ALIX rabbit antibody (ab186429; Abcam), and anti‐CYCS mouse antibody (sc‐13156; Santa Cruz Biotechnology) as well‐known EV markers. The secondary antibodies used were a horseradish peroxidase‐conjugated anti‐rabbit IgG (AP132P; Merck), anti‐rat IgG (81‐9520; Thermo Fisher Scientific), and anti‐mouse IgG antibodies (115‐035‐044; Jackson ImmunoResearch). Signals were visualized using an Immunostar LD Kit (FUJIFILM Wako Pure Chemical Corporation) and the C‐DiGit Blot Scanner and Image Studio for C‐DiGit (LI‐COR).
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