Dp21 cu 2 megapixel digital color camera

3T3-L1 originated adipocytes were stained with Nile red to identify lipid droplets, while macrophages with Hoechst 33342 (Thermo Fischer Scientific, 62249) to recognize macrophage nuclei and with 10 μM 5-carboxyfluorescein diacetate (CFDA) (Sigma-Aldrich, C4916) to recognize macrophage cytosol. Wild type or TG2 null macrophages were layered on top of the apoptotic adipocytes in a ratio of 5:1. The co-culture was placed in a temperature-, humidity- and CO₂-controlled, motorized Olympus IX-81 inverted microscope (Olympus America), which was equipped with a cooled Hamamatsu ORCA-R2 (Hamamatsu Photonics, Hamamatsu City, Japan) high-resolution monochrome CCD camera and a DP21-CU 2-megapixel digital color camera (Olympus America). Cells were monitored for 5 h and in every 5 min, an image was taken. Data were converted into a video file with the use of the Xcellence software (Olympus America). Lipid contents of adipocytes induced to undergo apoptosis by serum starvation were labeled by Nile red (Thermo Fisher Scientific, N1142).

Adipocytes were stained with Nile red and Hoechst 33342, macrophages were layered on top of the adipocytes in a ratio of 5 : 1. The coculture was placed in a temperature-, humidity- and CO₂-controlled, motorized Olympus IX-81 inverted microscope (Olympus America, Center Valley, PA, USA), which was equipped with a cooled Hamamatsu ORCA-R2 (Hamamatsu Photonics, Hamamatsu City, Japan) high-resolution monochrome CCD camera and a DP21-CU 2-megapixel digital color camera (Olympus). Cells were monitored for 15 h and in every 5 min an image was taken. Data were converted into a video file with the use of the Xcellence software (Olympus America).