Hiscript 2 q rt supermix

Manufactured by Selleck Chemicals

HiScript II Q RT Supermix is a reverse transcription reagent used for the conversion of RNA to cDNA. It contains optimized components for efficient and sensitive reverse transcription.

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Lab products found in correlation

Trizol, by Thermo Fisher Scientific (3 mentions) Sybr green, by Selleck Chemicals (3 mentions)

3 protocols using hiscript 2 q rt supermix

Profiling HNSCC Transcriptome Alterations

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Paired, fresh HNSCC tissues and their corresponding adjacent normal tissues were collected from the Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine. Written informed consent was obtained from each patient. The patients were well-informed and have consented to this study, and the process was approved by Ethics Committee of the Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine.(SH9H-2021-TK547-1).
Total RNA was extracted using TRIzol (Invitrogen, CA, United States). An equal amount of RNA was reverse-transcribed using the HiScript II Q RT Supermix and was quantified by qPCR using SYBR Green (Bimake). The primer sequences are shown in

Supplementary Table 1

Zhang X., Zhou W., Zhang Y, & Liu Z. (2022). CBX3 is a Prognostic Biomarker Correlated with ATR Activation and Immune Infiltration in Head and Neck Squamous Cell Carcinoma. International Journal of General Medicine, 15, 1497-1508.

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Quantitative RNA Expression Analysis

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Total RNA was extracted using TRIzol (Invitrogen, CA, United States). An equal amount of RNA was reverse- transcribed using the HiScript II Q RT Supermix and was quantified by qPCR using SYBR Green (Bimake). The primer sequences are shown in Supplementary Material.

Liu Z., Zhang Z., Zhang Y., Zhou W., Zhang X., Peng C., Ji T., Zou X., Zhang Z, & Ren Z. (2024). Spatial transcriptomics reveals that metabolic characteristics define the tumor immunosuppression microenvironment via iCAF transformation in oral squamous cell carcinoma. International Journal of Oral Science, 16, 9.

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RNA Extraction and Quantification

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Total RNA was extracted using TRIzol (Invitrogen, CA, United States). An equal amount of RNA was reverse-transcribed using the HiScript II Q RT Supermix and was quantified by qPCR using SYBR Green (Bimake). The primer sequences are shown in Supplementary Table 1.

Liu Z., Zhou W., Lin C., Wang X., Zhang X., Zhang Y., Yang R., Chen W, & Cao W. (2020). Dysregulation of FOXD2-AS1 promotes cell proliferation and migration and predicts poor prognosis in oral squamous cell carcinoma: a study based on TCGA data. Aging (Albany NY), 13(2), 2379-2396.

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