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Spectrofluorometer

Manufactured by Edinburgh Instruments

The Spectrofluorometer is a laboratory instrument designed to measure the fluorescence properties of samples. It excites the sample with a specific wavelength of light and records the emission spectrum, allowing the identification and quantification of fluorescent molecules within the sample.

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2 protocols using spectrofluorometer

1

Photophysical Characterization of Organic Compounds

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UV/Vis spectra were recorded with Shimadzu UV-2600 spectrophotometer. Photoluminescent spectra were recorded on a Varian Cary Eclipse spectrofluorometer. UV/Vis and fluorescence spectra of solutions were recorded using standard 1 cm quartz cells at room temperature. The ФF values were calculated using the established procedure with 3-aminophalimide in ethanol (ФF = 0.60) and in quinine sulfate 0.1N H2SO4F = 0.55) [47 ]. Fluorescence spectra in a solid state were measured by the integrating sphere Quanta-φ F-3029 at Horiba FluoroMax-4.
The emission lifetimes have been measured using the TCSPC option of FS5 Edinburgh Instruments spectrofluorometer. The sample has been excited by EPLED-300 picosecond pulsed light emitted diode centered at 300 nm and EPL-375 picosecond pulsed diode laser centered at 375 nm. The instrument response function (IRF) has been recorded under described conditions by replacing the sample with a silica diffuser. The time decay data have been analyzed by nonlinear least-squares fitting with the deconvolution of the IRF using the Fluoracle software package.
Analytical experiments were carried out by using equipment from the Center for Joint Use «Spectroscopy and Analysis of Organic Compounds» at the Postovsky Institute of Organic Synthesis of the Ural Branch of the Russian Academy of Sciences.
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2

Characterization of Organic Compounds

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General: Reagent grade chemicals were purchased from Sigma Aldrich and Acros Organics and used without further purification. Yields reported are for isolated, spectroscopically pure compounds. UV‐Vis spectra were measured at 20 °C on an Evolution 220 spectrometer equipped with a thermostat, using quartz cuvettes with a 10 mm path length. Fluorescence spectra were recorded at 20 °C on a Fluoromax 4 spectrometer from HORIBA equipped with a thermostat, using triangular quartz cuvettes with 10 mm catheti. Solid state emission spectra and quantum yields (integrating sphere) were measured on an Edinburgh Instruments spectrofluorometer. NMR spectra were recorded on a Bruker Advance DPX 300 spectrometer using TMS or the residual solvent proton as internal standard. HRMS spectra were recorded on FTMS 4.7T BioAPEX II and Waters Synapt G2‐Si.
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