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2 protocols using sodium nitrite

1

Analytical reagents for UPLC-MS

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Methanol, acetonitrile and formic acid were UPLC-MS-grade reagents purchased from CNW Technologies (Düsseldorf, Germany). Phenol, sulfuric acid, ethanol, aluminum nitrate (Al(NO3)3), sodium nitrite (NaNO2) and sodium hydroxide (NaOH) were analytical-grade reagents purchased from Xilong Scientific Co., Ltd., (Guangdong, China).
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2

Phytochemical Analysis of Botanical Compounds

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The solution of Gallic acid, folin-phenol and rutin were purchased from Shanghai Yuanye Biotechnology Co., ltd. The phenol was provided by Aladdin Biotechnology Co., ltd. (Shanghai, China). The hydrochloric acid, sulfuric acid, petroleum ether (30 ∼ 60 ℃), copper sullipide, potassium sullipide, boric acid, sodium nitrite, sodium carbonate, aluminum chloride, and sodium hydroxide were purchased from Xilong Scientific Co., ltd. (Shanghai, China). Standards of the cyanidin, delphinidin, petunidin, peonidin, pelargonidin, and malvidin (HPLC grade, percent purity was ≥ 99 %) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). All other chemicals and solvents were of analytical grade.
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