Plasma concentrations of cortisol were determined by a Cortisol Express EIA Kit (AYN830, Cayman Chemical, Ann Arbor, MI, USA). The limit of sensitivity of the assay was 0.1 ng·mL−1.
Cortisol express eia kit
Manufactured by Cayman Chemical
Sourced in United States
The Cortisol Express EIA Kit is a laboratory assay designed to measure cortisol levels in various sample types. It provides a quantitative analysis of cortisol concentration using an enzyme immunoassay (EIA) technique.
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Lab products found in correlation
4 protocols using cortisol express eia kit
1
Cortisol Quantification by EIA
2
Cortisol Measurement in Zebrafish
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In view of the circadian rhythms, zebrafish were anesthetized with MS-222 at 9:00 am. For 6 dpf larval zebrafish, a pool of 30 larvae were homogenized in 500 μl cold PBS and then homogenized. At least eight 30-fish pools of each genotype were measured. Plasma was collected from 150 dpf zebrafish. The cortisol content was measured with a cortisol express EIA kit (Item No. 500370) from Cayman Chemical Company following the procedure provided by the manufacturer.
3
Plasma Cortisol Measurement Protocol
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To determine the cortisol level in the plasma, Cortisol Express EIA Kit (Cayman, USA) was used.
4
Quantifying Plasma Hormones: Leptin, Cortisol, and Insulin
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Plasma concentrations of leptin were determined by a multi-species leptin RIA kit (XL-85K, Millipore, Molsheim, France). The limit of sensitivity of the assay was 2 ng·mL−1 and the inter- and intra-assay coefficients of variance were <9 and <4%, respectively.
Plasma concentrations of cortisol were determined by a Cortisol Express EIA Kit (AYN830, Cayman Chemical, Ann Arbor, MI, USA). The limit of sensitivity of the assay was 0.1 ng·mL−1, while the coefficients of variance for a dose of 5 ng were equal, respectively, to 11 and 6% for inter- and intra-assays. Plasma levels of insulin were assayed with Ultra Rat Insulin ELISA Kit (# 90060, Crystal Chem, Downers Grove, IL, USA) using hamster insulin standard (# 90330, Crystal Chem) following the instructions of the manufacturer. The limit of sensitivity of the assay was 0.1 ng·mL−1 and the coefficients of variance were <10% for both inter- and intra-assays.
Plasma concentrations of cortisol were determined by a Cortisol Express EIA Kit (AYN830, Cayman Chemical, Ann Arbor, MI, USA). The limit of sensitivity of the assay was 0.1 ng·mL−1, while the coefficients of variance for a dose of 5 ng were equal, respectively, to 11 and 6% for inter- and intra-assays. Plasma levels of insulin were assayed with Ultra Rat Insulin ELISA Kit (# 90060, Crystal Chem, Downers Grove, IL, USA) using hamster insulin standard (# 90330, Crystal Chem) following the instructions of the manufacturer. The limit of sensitivity of the assay was 0.1 ng·mL−1 and the coefficients of variance were <10% for both inter- and intra-assays.
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