P38 mapk antibody

The concentration of extracted protein was measured by BCA method. The protein bands were isolated by SDS-PAGE gel electrophoresis and transferred into PVDF membrane (0.45 µm). At room temperature, the PVDF membrane containing the target protein was immersed in the Blotting-Grade blocker (BIO-RAD, California, USA) for 2 h. After blocking, wash three times with buffer solution (TBST). At 4 ℃, the specific primary antibody was incubated overnight. They were washed with buffer solution (TBST) and then incubated with the second antibody for 4 h at room temperature. CD81, CD63 and Phospho-p38 MAPK antibody are rabbit-derived polyclonal antibody purchased from Affinity Biosciences. p38 MAPK antibody, MK2 antibody and β-actin antibody are mouse-derived monoclonal antibodies purchased from Proteintech Group. Secondary antibodies are goat anti-rabbit HRP (Affinity Biosciences) and goat anti-mouse HRP (Proteintech Group), respectively. After washing with buffer solution for three times, test results were obtained by gel imaging system (BIO-RAD ChemiDoc XRS +). Images and results were quantified with Image J software.

Human adiponectin (Acrp30) and human TGF-β1 were purchased from Novoprotein (Beijing, China). Cell Counting Kit-8 (CCK-8) and EdU kits were procured from SolarBio (Beijing Solebo Technology Co., Ltd, Beijing, China). PCNA antibody, smooth muscle actin polyclonal (α-SMA) antibody, collagen type I (collagen I) rabbit polyclonal antibody, p38 MAPK antibody, p-p38 MAPK antibody, NF-κB antibody, p-NF-κB antibody, β-catenin antibody, p-β-catenin antibody, Notch1 antibody, and HRP-conjugated AffiniPure goat anti-mouse IgG (H + L) were obtained from Proteintech (Wuhan, China). SB203580 was purchased from MCE (Shanghai, China). Other chemicals used in this study, such as the BCA protein assay kit and SDS-PAGE gel separation kit, were acquired from ASPEN (ASPEN Biotechnology Co., Ltd, Wuhan, China).