Sc300 infrared camera

The size and morphology of HSA@CySCOOH was investigated by atomic force microscopy (AFM). After suitable sample absorption on freshly peeled mica substrate, rinsing, drying, and dehumidification, biological AFM imaging of the HSA or HSA@CySCOOH samples was carried out in air, using gentle tapping-mode AFM with a PicoForce Multimode AFM (Bruker, CA) consisting of a Nanoscope® V controller, a type E scanner head, and a sharpened TESP-SS (Bruker, CA) or similar AFM cantilever. AFM images were evaluated within the Nanoscope software (ver. 7.3–8.15, Bruker, CA) and exported to Image J (ver. 1.4×, National Institutes of Health, MD) for further analyses and display.
UV/Vis spectra were measured by a Genesys 10S UV-Vis spectrophotometer (Thermo Scientific, Waltham, MA) using quartz cuvettes with an optical path of 1 cm. Fluorescence intensity was monitored with an F-7000 fluorescence spectrophotometer (Hitachi, Tokyo, Japan). Thermal images were captured by a SC300 infrared camera (FLIR, Arlington, VA) and processed with Examin IR image software (FLIR). The excitation source was an 808 nm diode-pumped solid-state laser system (LASERGLOW Technologies, Toronto, Canada).

Thermal images were captured by a SC300 infrared camera (FLIR, Arlington, VA). The images were analyzed by Examin IR image software (FLIR). The excitation source was an 808 nm diode-pumped solid-state laser system (LASERGLOW Technologies, Toronto, Canada). Thermal images of HeLa-tumor-bearing mice were taken at 24 and 48 h p.i. (1 at 5 mg/kg, and 2 at 100 mg/kg, n = 5) with exposure to 808 nm laser irradiation (0.8 W/cm², 5 min).