Poloxamer 407 pluronic f 127

The MNs were fabricated using the biocompatible Class I acrylic resin, Dental SG (Formlabs, GoPrint3D, North Yorkshire, UK). Insulin derived from bovine pancreas (10 mg mL⁻¹) was acquired from Sigma-Aldrich (Gillingham, UK). Polyvinylpyrrolidone (PVP, Kollidon 17PF) and Poloxamer 407 (Pluronic F-127) were purchased from BASF (London, UK). All solvents were of analytical grades.

Amphotericin B, as the active pharmaceutical ingredient, was bought from Bio Basic Inc. (Toronto, ON, Canada). Intravenous Amphotericin B was supplied by Biolab (Samutprakarn, Thailand). Polyethylene glycol 400 (PEG) was purchased from Nam Siang Co. Ltd. (Bangkok, Thailand). Branched polyethylenimine (PEI), hyaluronic acid, mucin from porcine stomach type II, hydrocortisone, insulin from bovine pancreas, and fluorescein isothiocyanate were ordered from Sigma-Aldrich (St. Louis, MO, USA). Poloxamer 407 (Pluronic^® F127) was acquired from BASF (Florham Park, NJ, USA). Muller-Hinton agar and Sabouraud Dextrose agar were purchased from HIMEDIA (Mumbai, India). Roswell Park Memorial Institute (RPMI) 1640 medium, keratinocyte serum free medium (K-SFM), bovine pituitary extract (BPE), epithelial growth factor (EGF), and Penicillin/ Streptomycin solution were ordered from Gibco (New York, NY, USA). Thiazolyl blue tetrazolium bromide was acquired from Amresco^® (Solon, OH, USA). Crystal violet was supplied by Riedel-de Haën (Munich, Germany). All other reagents were of analytical grade or higher.

Caspofungin acetate salt (molecular weight ~1200 Da) was acquired from SunPharma (Barcelona, Spain). Poloxamer 407 (Pluronic F-127, molecular weight ~12,500 Da) was supplied by BASF (Barcelona, Spain), chitosan medium molecular weight (190–310 KDa and deacetylation degree ≥ 75%) was purchased from Sigma Aldrich (Madrid, Spain) and diethylene glycol monoethyl ether (Transcutol-P, molecular weight ~130 Da) was provided by Gattefossé (Saint-Priest, France). Azone (molecular weight ~280 Da) was acquired from Netqem (Durham, NC, USA), and acetic acid and reagents for the analytical method were acquired from Panreac (Barcelona, Spain). Purified and filtered water was obtained using a Milli-Q^® Gradient A10 system apparatus (Millipore Iberica SAU.; Madrid, Spain).