Mouse whole blood was harvested by cardiac puncture and plasma and serum were isolated using lithium heparin-coated Microtainer blood collection tubes (BD, 365965) and Microtainer blood collection tubes (BD, 365978), respectively. For flow cytometric, bead-based immunoassays, plasma was diluted and processed using the LEGENDplex Mouse Inflammation Panel (BioLegend, 740446) and Mouse Macrophage/Microglia Panel (BioLegend, 740846) kits. Data were acquired on the NovoCyte flow cytometer (Acea Biosciences/Agilent) and analyzed using the LEGNEDplex software v8 (BioLegend). To measure tissue injury marker levels in sera, samples were processed with the following kits for BUN (BioAssay Systems DIUR-100), ALT (Cayman Chemical, 700260) and troponin-I (Life Diagnostics, CTNI-1-HS) levels according to the manufacturer’s instructions.
Ctni 1 hs
Manufactured by Life Diagnostics
The CTNI-1-HS is a lab equipment product designed for the quantitative measurement of cardiac troponin I (cTnI) levels. It serves as a tool for the assessment of myocardial injury or dysfunction. The core function of the CTNI-1-HS is to provide accurate and reliable cTnI concentration data, which can assist healthcare professionals in diagnostic and treatment decisions.
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Lab products found in correlation
3 protocols using ctni 1 hs
1
Comprehensive Blood Characterization Protocol
2
Measuring Urinary and Plasma Corticosterone
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For urinary corticosterone measurements, all urine was collected from mice over a 24 h period. Corticosterone was then measured by ELISA (Arbor Assays: K014-HI). To control for differences in overall urine concentration, urinary creatinine was measured by ELISA (Enzo Life Sciences: ADI-907-030A). A ratio of corticosterone:creatinine was then generated.
For plasma corticosterone measurements, tail blood or trunk blood following mouse decapitation was collected and immediately placed at 4 °C. Blood was centrifuged at 20,000 rcf at 4 °C and plasma was frozen at −20 °C. Plasma corticosterone was determined by I-125 radioimmunoassay (MP Biomedical, Solon, OH, USA) [26] (link). The intra-assay and inter-assay CVs for plasma corticosterone were 7.6% and 13.3%, respectively. Serum cardiac troponin I (cTnI) was measured by ELISA (Life Diagnostics: CTNI-1-HS).
For plasma corticosterone measurements, tail blood or trunk blood following mouse decapitation was collected and immediately placed at 4 °C. Blood was centrifuged at 20,000 rcf at 4 °C and plasma was frozen at −20 °C. Plasma corticosterone was determined by I-125 radioimmunoassay (MP Biomedical, Solon, OH, USA) [26] (link). The intra-assay and inter-assay CVs for plasma corticosterone were 7.6% and 13.3%, respectively. Serum cardiac troponin I (cTnI) was measured by ELISA (Life Diagnostics: CTNI-1-HS).
3
Mouse Serum Cardiac Troponin-I Quantification
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Retro-orbital bleeds of anesthetized mice were collected in 1.5 mL tubes and centrifuged at 300 xg for 10 minutes. The transparent serum layer above the RBC pellet was removed and stored as single-use 100 μL aliquots at -80°C. Levels of cardiac troponin-I in the serum were measured by high-sensitivity ELISA using manufacturer’s protocol (Life Diagnostics, West Chester, PA; cat # CTNI-1-HS).
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