Cholesta 3 5 diene

Ethanolamine [1-³H] hydrochloride (40 Ci/mmol) was purchased from the American Radiolabeled Chemicals, Inc. Choline [methyl-¹⁴C] chloride (52 mCi/mmol) and [α-³²P]-dCTP (3000 Ci/mmol) were purchased from Perkin Elmer. Phospholipid standards for mass spectrometry studies including 1-stearoyl-2-arachidonoyl-sn-glycero-3-phosphoethanolamine (18:0-20:4-PE), 1-(1Z-octadecenyl)-2-arachidonoyl-sn-glycero-3-phosphoethanolamine (p18:0-20:4-PE), and 1,2-dimyristyl-sn-glycero-3-phosphocholine (14:0-14:0-PC) were purchased from Avanti Polar Lipids. Cholesta-3,5-diene was purchased from Sigma Aldrich as the standard for sterol analysis. The anti-LPG monoclonal antibody was acquired from the culture supernatant of mouse hybridoma WIC79.3 cells (de Ibarra et al., 1982 (link)). The mouse anti-Leishmania GP63 monoclonal antibody (for immunofluorescence microscopy) was purchased from the Cedarlane Laboratories. The mouse-anti-α-tubulin, rabbit-anti-GFP, goat anti-rabbit IgG-HRP, goat anti-mouse IgG-HRP, and goat anti-mouse IgG-FITC antibodies were purchased from Life Technologies. All other reagents were purchased from VWR International or Fisher Scientifics unless specified otherwise.

Cholesta-3,5-diene (FW: 368.65) was purchased from Sigma-Aldrich (St. Louis, MO) as an internal standard for the quantification of sterols in GC-MS chromatograms. Itraconazole (ITZ) was purchased from LKT Laboratories, Inc. (St. Paul, MN). Amphotericin B (Amp B) was purchased from EMD Chemicals, Inc. (San Diego, CA). Myriocin was purchased from Sigma-Aldrich (St. Louis, MO). 1-(4-Trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene p-toluenesulfonate (TMA-DPH), MitoTracker™ Red CMXRos, tetramethylrhodamine ethyl ester (TMRE), dihydroethidium and MitoSox Red were purchased from Thermo Fisher Scientific (Waltham, MA).
BALB/c and C57BL6 (female, 7–8 weeks old) mice were purchased from Charles River Laboratories International (Wilmington, MA). All procedures involving mice were carried out according to protocols approved by the Animal Care and Use Committee at Texas Tech University (PHS Approved Animal Welfare Assurance No. A3629–01). Mice were housed and cared for in the facility operated by the Animal Care and Resources Center at Texas Tech University adhering to the Guide for the Care and Use of Laboratory Animals (the 8th Edition, NRC 2011) for animal husbandry.