Coreldraw 2012

Digital microphotographs from chromogenic experiments were taken on a Leica microscope (DMR HC, Leica Microsystems GmbH) equipped with a Zeiss Axiovision Digital Camera (Carl Zeiss, Germany), using ×1.6 and ×5 magnification objectives. Serial images from fluorescent material were taken with a confocal microscope (Olympus FV1000, Olympus Corporation, Japan) using ×10 and ×40 objectives; Z-series stacks were taken at 2 μm-step to allow analysis of co-expression. The fluorescent images were adjusted for brightness and contrast, and extracted using Olympus FV10-ASW 4.2 Viewer (Olympus Corporation). Some of the images at ×40 were used to estimate degree of colocalization in selected areas. Counting was done manually using ImageJ Fiji (Schindelin et al., 2012 (link)) at a single z-level, selected for displaying the best labeling for all markers. All the figures were mounted using CorelDraw 2012 (Corel Corporation, Canada).

Digital microphotographs from conventional in situ hybridization and immunohistochemistry were obtained with a Leica microscope (DMR HC; Leica Microsystems GmbH, Germany) equipped with a Zeiss Axiovision Digital Camera (Carl Zeiss, Germany). Serial images from a fluorescent material were taken with a confocal microscope (Olympus FV1000; Olympus Corporation, Japan). Selected digital immunohistochemical images were adjusted for brightness and contrast with Corel PHOTO-PAINT 2012 (Corel Corporation, Canada), while fluorescent images were adjusted and extracted using Olympus FV10-ASW 4.2 Viewer (Olympus Corporation). Finally, figures were mounted using CorelDraw 2012 (Corel Corporation).