HPLC-grade methanol, ammonium bicarbonate, Optima™ acetone, heat sealing foil, 96-well deep well plates (Fisher Scientific; Hanover, IL); Optima™ LC/MS-grade formic acid (Sigma-Aldrich; St. Louis, MO); proteinase K isolated from Tritirachium album (Millipore; Billerica, MA); HPLC-grade water and acetonitrile (Tedia; Fairfield, OH); 96-well PCR plates and adhesive PCR foil (Eppendorf; Hauppauge, NY); Kinetex C18 column (2.1 x 50 mm, 1.3 µm) (Phenomenex; Torrance, CA); Oasis Hydrophilic/Lipophilic Balanced (HLB) 96-well (30 mg) SPE plates (Waters; Milford, MA); Environmental Protection Agency ultra-dilute sulfur mustard (10 µg/mL) reference standard in isopropanol (Lawrence Livermore National Laboratory; Livermore, CA).
Optima lc ms grade formic acid
Manufactured by Merck Group
Sourced in United States
Optima™ LC/MS-grade formic acid is a high-purity reagent used in liquid chromatography-mass spectrometry (LC/MS) applications. It is designed to provide consistent performance and minimize background interference in sensitive analytical techniques.
Automatically generated - may contain errors
2 protocols using optima lc ms grade formic acid
1
Comprehensive Metabolite Extraction and Analysis
2
Preparation and Analysis of Metabolite Standards
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Metabolite compound standards were obtained from Sigma-Aldrich (St. Louis, MO, USA), Steraloids Inc. (Newport, RI, USA) and TRC Chemicals (Toronto, ON, Canada). The stock solution for each compound was prepared by dissolving the individual standard in water, methanol, sodium hydroxide, or hydrochloric acid at a concentration of 5.0 mg/mL, and calibration stock solution was made by mixing appropriate amount of each stock solution. Optima LC/MS grade formic acid was obtained from Sigma-Aldrich (St. Louis, MO, USA), and Optima LC/MS grade methanol, acetonitrile, and isopropanol were purchased from Thermo-Fisher Scientific (FairLawn, NJ, USA). Ultrapure water was produced by a Mill-Q Reference system equipped with a LC-MS Pak filter (Millipore, Billerica, MA, USA).
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