Rnalater rna stabilization

The samples were stored in RNAlater RNA Stabilization (Qiagen, Germany) solution. RNeasy Mini Kit columns were used to extract total RNA according to the manufacturer’s protocol (Qiagen). In order to measure RNA quality, an Agilent 2100 Bioanalyzer using the RNA 6000 Nano Chip (Agilent Technologies, Amstelveen, The Netherlands) was used. Integrity values to RNA measurement were confirmed by the RNA integrity number (RIN). Total RNA quantity was assessed with Infinite 200 PRO NanoQuant Spectrophotometer (TECAN, Switzerland).

Genomic DNA and total RNA were extracted from a single surgical sample (one per patient) that contained primary lung adenocarcinoma and adjacent noncancerous lung tissue. Samples were snap-frozen or stored in RNAlater RNA Stabilization (Qiagen, Germany) solution. Total RNA was isolated using RNeasy Mini Kit columns according to the manufacturer's protocol (Qiagen). RNA quality was determined using the RNA integrity number and was assessed on an Agilent 2100 Bioanalyzer using an RNA6000 Nano Chip (Agilent Technologies, CA, USA). Total RNA quantity was determined using an Infinite 200 PRO NanoQuant Spectrophotometer (TECAN, Switzerland). Genomic DNA was extracted from tissues according to the QIAamp DNA mini kit protocol (Qiagen).