Anti-Myc (9E10), anti-His (H-15), anti-GAPDH (0411) and anti-HIF-1α (H206) antibodies were purchased from Santa Cruz. Anti-hemagglutinin (anti-HA) antibody was purchased from Covance. Anti-flag (F1804) antibody was purchased from Sigma. Anti-α-tubulin (EPR1333) and anti-Set7 (EPR5574) antibodies were purchased from Epitomics. Anti-HIF-1α (NB100–105) was purchased from Novus Biologicals. Anti-SOD2 (EPR2560Y) antibody was purchased from GeneTex. Anti-PAI-1 (612025) antibody was purchased from BD Transduction Laboratories. Anti-Histone H3 [(D1H2)XP] antibody was purchased from Cell Signaling. Anti-HIF-1α(CH3)-K32 polyclonal antibody was obtained at Abmart with the HIF-1α-K32-me1 peptide (Shanghai, China). The active Set7 protein (14–469) was purchased from Millipore, and S-adenosylmethionine (SAM) (B903S) was purchased from New England Biolabs (NEB). 2ME2 (S1233) was purchased from Selleck, and (R)-PFI-2 was obtained from Tocris. Glucose assay kit was purchased from BioVision, and adenosine triphosphate (ATP) assay kit from Beyotime. 6-[N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxy-D-glucose(2-NBDG), a glucose analog, was purchased from Invitrogen.
Anti hif 1α h206
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Anti-HIF-1α (H206) is a monoclonal antibody that specifically recognizes the hypoxia-inducible factor-1 alpha (HIF-1α) protein. HIF-1α is a transcription factor that plays a central role in the cellular response to hypoxia. This antibody can be used for the detection and quantification of HIF-1α in various experimental applications.
Automatically generated - may contain errors
Lab products found in correlation
Adenosine triphosphate atp assay kit, by Beyotime (1 mentions)
6 n 7 nitrobenz 2 oxa 1 3 diazol 4 yl amino 2 deoxy d glucose 2 nbdg, by Thermo Fisher Scientific (1 mentions)
Anti flag antibody f1804, by Merck Group (1 mentions)
Glucose assay kit, by Abcam (1 mentions)
R pfi 2, by Bio-Techne (1 mentions)
Anti hif1α nb100 105, by Novus Biologicals (1 mentions)
Anti γ tubulin c 20, by Santa Cruz Biotechnology (1 mentions)
Anti e cadherin, by Cell Signaling Technology (1 mentions)
Anti fth, by Santa Cruz Biotechnology (1 mentions)
Anti ha probe f 7, by Santa Cruz Biotechnology (1 mentions)
Anti snail, by Cell Signaling Technology (1 mentions)
Anti vimentin, by Cell Signaling Technology (1 mentions)
Anti slug, by Cell Signaling Technology (1 mentions)
Anti cxcr4, by Abcam (1 mentions)
Ecl western blotting detection system, by Bio-Rad (1 mentions)
2 protocols using anti hif 1α h206
1
Biochemical Characterization of HIF-1α Regulation
2
Western Blot Analysis of Protein Targets
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A total of 40 μg protein extract was boiled for 10 min in SDS sample buffer, separated by 12% SDS-PAGE and transferred to a nitrocellulose membrane by electroblotting as reported in Di Sanzo et al. (38 (link)). The nitrocellulose membranes were incubated overnight at 4°C with the following antibodies: (a) anti-CXCR4 (1:500; Abcam), (b) anti-HIF-1α (H-206) (1:200; Santa Cruz Biotechnology), (c) anti-p65 (C-20) (sc-372, 1:1,000; Santa Cruz Biotechnology), (d) anti-HDAC (1:5,000; Sigma-Aldrich), (e) anti-HA probe (F-7) (1:1,000; Santa Cruz Biotechnology), (f) anti-Vimentin, (g) anti-E-cadherin, (h) anti-Snail, (i) anti-Slug (1:1,000; Cell Signaling Technology, Danvers, MA, USA), (l) anti-FtH (1:200; Santa Cruz Biotechnology), (m) anti-γ-Tubulin (C-20) (1:2,000; Santa Cruz Biotechnology), (n) anti-Nucleolin (D4C7O) (1:1,000; Cell Signaling Technology) over-night at 4°C, followed by incubation with goat anti-rabbit and mouse anti-goat secondary antibodies (1:5,000; Santa Cruz Biotechnology). Membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies and immunoreactive bands were visualized with the ECL Western blotting detection system (BioRad, Hercules, CA, USA).
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