Stock cultures, including methicillin-resistant Staphylococcus aureus (MRSA), methicillin-sensitive Staphylococcus aureus (MSSA), Staphylococcus epidermidis, Escherichia coli, Candida albicans, and Pseudomonas aeruginosa, were used for antimicrobial activities evaluation of TA free in solution and in CMTA. Test organisms were first activated from glycerol by transfer in nutrient broth at 37 °C for 24 h, then streaking on Mueller–Hinton agar (MHA) (Sigma-Aldrich, USA). A single pure colony was streaked on MHA and incubated at 37 °C for 24 h. Then the concentrations of microorganisms were adjusted with the turbidity of 0.5 McFarland (equal to 1.5 × 108 colony-forming units (CFU)/mL). Turbidity of the microbial suspensions were prepared in sterile saline solution and measured at 600 nm using a mini 1240 UV-Vis spectrophotometer (Shimadzu Corp., Kyoto, Japan), followed by the experiment.
Mini 1240 uv vis spectrophotometer
Manufactured by Shimadzu
Sourced in Japan
The Mini 1240 UV-Vis spectrophotometer is a compact and lightweight instrument designed for basic absorbance measurements in the ultraviolet and visible wavelength ranges. It features a wavelength range of 190-1100 nm and can be used for a variety of applications that require accurate and reliable absorbance data.
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Lab products found in correlation
5 protocols using mini 1240 uv vis spectrophotometer
1
Antimicrobial Activities Evaluation of TA
2
Comprehensive Beverage Nutrient Analysis
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The total protein in the beverage samples was determined using the Biuret method as described by Keppy & Allen (2009) , and the absorbance of the samples and standards was measured at 540 nm using a mini 1240 UV-VIS spectrophotometer (Shimadzu, Kyoto, Japan). The protein contents in the samples were calculated using the linear equation (r 2 = 0.996) generated using standards with known protein concentrations. The total carbohydrate contents were determined using a phenol-sulfuric acid colorimetric method as described previously (Nielsen, 2010) (link). The total fat was determined using a gravimetric method described by Phillips et al. (1997) (link).
3
Physicochemical and Microbial Analysis of Date Palm Spathe Beverages
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The TDS and TNU in the beverage samples were also assessed for the developed date palm spathe-based beverages according World Health Organization (1996) guidelines for drinking-water quality, TDS measured using an ORION 5-STAR pH ISE conductivity meter with a conductivity cell (Thermo Scientific, London, UK). The turbidity (NTU) was measured using spectrophotometrically (Mini 1240UV-VIS spectrophotometer Shimadzu, Kyoto, Japan).
All beverages were analysed for microbial load (bacterial counts, Escherichia coli, moulds and yeasts) according to American Public Health Association (1992) and Kang et al. (2003) (link). using VIDAS and Biomerieux (Paris, France) microbial analysis tools. Media selective for each microbe was used, and the cultures were incubated at 35 °C, 30 °C, and 42 °C for 24 h for coliform bacteria, E. coli, and Salmonella, respectively, whereas that of yeast and moulds were incubated at 25 °C for 3 d. Results were expressed as CFU g -1 .
All beverages were analysed for microbial load (bacterial counts, Escherichia coli, moulds and yeasts) according to American Public Health Association (1992) and Kang et al. (2003) (link). using VIDAS and Biomerieux (Paris, France) microbial analysis tools. Media selective for each microbe was used, and the cultures were incubated at 35 °C, 30 °C, and 42 °C for 24 h for coliform bacteria, E. coli, and Salmonella, respectively, whereas that of yeast and moulds were incubated at 25 °C for 3 d. Results were expressed as CFU g -1 .
4
Spectrophotometric Quantification of MDA
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Malondialdehyde (MDA) was extracted from minced steaks in duplicate, as performed by Selani et al. [27 (link)]. For quantification, the absorbance of the extracts was measured with a UV–Vis mini 1240 spectrophotometer (Shimadzu, Kyoto, Japan) at 532 and 600 nm against a blank of ultrapure water. A standard curve was prepared with 1,1,3,3-tetraethoxypropane (from 0 to 0.4 nmol/L), and the thiobarbituric acid reactive substances (TBARS) content was expressed as mg of MDA/kg of sample.
5
Anabaena 7120 Cell Density and Chlorophyll a Quantification
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The cell density was calculated using light absorption at 730 nm (A730) in a UV-Vis mini-1240 spectrophotometer (SHIMADZU, Kyoto, Japan) with a 3 mL cuvette and an optical path of 1 cm. Chlorophyll a (Chl a) content was measured as previously described48 . In summary, Anabaena 7120 cells (1.5 mL) were collected by centrifugation and washed twice with 1 mL of fresh BG-II medium. The supernatant was discarded after centrifugation; 1.5 mL of methyl alcohol was added to the cell pellet; and the mixture was incubated at 4°C for 6–14 h. The supernatant was then collected by centrifugation and absorbance at 665 nm (A665) was measured. The concentration of Chl a (μg mL−1) was calculated as 13.9 × A66549 (link).
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