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Cytotune kits

Manufactured by Thermo Fisher Scientific
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The CytoTune kits are a set of reagents and protocols designed to reprogram somatic cells into induced pluripotent stem cells (iPSCs). The kits use Sendai virus vectors to deliver the necessary transcription factors required for reprogramming.

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CytoTune-iPS 2.0 Sendai Reprogramming Kit (250 citations) CytoTune-iPS Sendai Reprogramming Kit (46 citations) CytoTune (22 citations) CytoTune®-iPSC Sendai Reprogramming Kit (9 citations) CytoTune-iPS 2.0 Sendai Virus Reprogramming Kit (5 citations) CytoTune-iPS 2.0 Sendai kit (3 citations) Cytotune 2 (A16517) virus kit (2 citations) OSKM CytoTune-iPS 2.0 Sendai Reprogramming kit viral particle factors (2 citations) CytoTune-iPS 1.0 Reprogramming kit (2 citations) CytoTune iPS 2.0 kit (2 citations)

2 protocols using cytotune kits

1

Generating iPSCs from Familial Alzheimer's Fibroblasts

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The control iPSC lines were generously provided by Dr. Gary Bassell’s laboratory from Emory University. The fAD fibroblasts were generously provided by Dr. Selina Wray, from UCL Queen Square Institute of Neurology and then generated into fAD iPSCs by Dr. Chadwick Hales, from Emory University. Fibroblasts and iPS cells were collected and stored under Emory IRB approved protocol IRB00064365. iPSCs were generated using the Invitrogen CytoTune kits with Sendai virus and Yamanaka factors. Samples were maintained in standard growth conditions or cryopreserved in liquid nitrogen for longer term storage.
Kuehner J.N., Chen J., Bruggeman E.C., Wang F., Li Y., Xu C., McEachin Z.T., Li Z., Chen L., Hales C.M., Wen Z., Yang J, & Yao B. (2021). 5-hydroxymethylcytosine is dynamically regulated during forebrain organoid development and aberrantly altered in Alzheimer’s disease. Cell reports, 35(4), 109042.
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2

Generating iPSCs from Familial Alzheimer's Fibroblasts

Check if the same lab product or an alternative is used in the 5 most similar protocols
The control iPSC lines were generously provided by Dr. Gary Bassell’s laboratory from Emory University. The fAD fibroblasts were generously provided by Dr. Selina Wray, from UCL Queen Square Institute of Neurology and then generated into fAD iPSCs by Dr. Chadwick Hales, from Emory University. Fibroblasts and iPS cells were collected and stored under Emory IRB approved protocol IRB00064365. iPSCs were generated using the Invitrogen CytoTune kits with Sendai virus and Yamanaka factors. Samples were maintained in standard growth conditions or cryopreserved in liquid nitrogen for longer term storage.
Kuehner J.N., Chen J., Bruggeman E.C., Wang F., Li Y., Xu C., McEachin Z.T., Li Z., Chen L., Hales C.M., Wen Z., Yang J, & Yao B. (2021). 5-hydroxymethylcytosine is dynamically regulated during forebrain organoid development and aberrantly altered in Alzheimer’s disease. Cell reports, 35(4), 109042.
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