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Anti phospho akt s473

Manufactured by Thermo Fisher Scientific

The Anti-phospho–AKT (S473) is a laboratory reagent used to detect and quantify the phosphorylation of the AKT protein at the serine 473 residue. It is a specific antibody that can be used in various analytical techniques, such as Western blotting, immunohistochemistry, and flow cytometry, to study the activation and signaling pathways of the AKT protein.

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2 protocols using anti phospho akt s473

1

Dextran Sulfate Sodium-Induced Colitis Model

Check if the same lab product or an alternative is used in the 5 most similar protocols
DSS (MW 47,000; MP Biomedicals) was purchased from MP Biomedicals. LPS (Escherichia coli 0111:B4), Percoll, and 4-PBA were from Sigma-Aldrich. Anti-phospho–AKT (S473) and anti-phospho–SRC (Tyr418) were from eBioscience. Anti-phospho–STAT5, anti-phospho–STAT1, and anti-phospho–spleen tyrosine kinase (SYK) Ab were from Cell Signaling Technology. Anti-phospho–AMP-activated protein kinase (AMPK) and anti-PPARγ Abs were purchased from Bioss Antibodies. Conjugated Abs with fluorescent dyes against Ly6G, CD11b, ICAM-1, CD29, CD244, and CD88 were from BioLegend. Anti-myeloperoxidase (MPO) Ab was from Abcam. G-CSF was from PeproTech. 3, 3′-diaminobenzidine was from Vector Laboratories.
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2

Dextran Sulfate Sodium-Induced Colitis Model

Check if the same lab product or an alternative is used in the 5 most similar protocols
DSS (MW 47,000; MP Biomedicals) was purchased from MP Biomedicals. LPS (Escherichia coli 0111:B4), Percoll, and 4-PBA were from Sigma-Aldrich. Anti-phospho–AKT (S473) and anti-phospho–SRC (Tyr418) were from eBioscience. Anti-phospho–STAT5, anti-phospho–STAT1, and anti-phospho–spleen tyrosine kinase (SYK) Ab were from Cell Signaling Technology. Anti-phospho–AMP-activated protein kinase (AMPK) and anti-PPARγ Abs were purchased from Bioss Antibodies. Conjugated Abs with fluorescent dyes against Ly6G, CD11b, ICAM-1, CD29, CD244, and CD88 were from BioLegend. Anti-myeloperoxidase (MPO) Ab was from Abcam. G-CSF was from PeproTech. 3, 3′-diaminobenzidine was from Vector Laboratories.
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