Osteopontin

Manufactured by Merck Group

Osteopontin is a glycoprotein found in various tissues, including bone, kidneys, and the immune system. It plays a role in biomineralization and cell signaling processes. Osteopontin can be used as a laboratory reagent for research purposes.

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Lab products found in correlation

Fibrinogen, by Enzyme Research (3 mentions) Fibronectin, by Enzyme Research (3 mentions) Ab58610, by Abcam (1 mentions) Ab8488, by Abcam (1 mentions) Ab51317, by Abcam (1 mentions) Ab16667, by Abcam (1 mentions) Sc 11427, by Santa Cruz Biotechnology (1 mentions) Ab6640, by Abcam (1 mentions) Alexa orange red 546 nm, by Thermo Fisher Scientific (1 mentions) 402oa 05a, by Merck Group (1 mentions) Chondrocyte growth medium, by PromoCell (1 mentions) Vitronectin, by Merck Group (1 mentions) Fibronectin, by Merck Group (1 mentions) Collagen 2, by Merck Group (1 mentions) Collagen 3, by Merck Group (1 mentions) Collagen 1, by Merck Group (1 mentions) Collagen 4, by Merck Group (1 mentions) Elisa plate, by Greiner (1 mentions)

Close spelling variants of this product

Anti-osteopontin Human Osteopontin ELISA Kit

3 protocols using osteopontin

Osteoarthritis Synovial Fluid Analysis and Chondrocyte Treatment

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Synovial fluid (1-1.5 ml) was extracted from the affected site of 58 OA patients (32 cases of knee and 26 cases of hip) before the initiation of therapy. Collection of synovial fluid samples was performed using a syringe under local anesthesia. Synovial fluid (1-1.5 ml) was also extracted from the knee of 32 healthy controls and the hip of 26 healthy controls to match OA group. All synovial fluid samples were immediately transferred to a liquid nitrogen sink for long-term storage.
Cell model used in this study were primary chondrocytes from OA patients (402OA-05A, Sigma-Aldrich, USA). Chondrocytes were cultivated with Chondrocyte Growth Medium (PromoCell) in a 5% CO₂ incubator at 37 °C. Subculture was performed at a ratio of 1:8. Cells used in subsequent experiments were collected at passage 1–2. In cases of osteopontin treatment, osteopontin (Sigma-Aldrich) was used to treat chondrocytes at dosages of 0, 1, 5 and 10 µg/ml for 48 h before use.

Liang Q., Asila A., Deng Y., Liao J., Liu Z, & Fang R. (2021). Osteopontin‐induced lncRNA HOTAIR expression is involved in osteoarthritis by regulating cell proliferation. BMC Geriatrics, 21, 57.

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Multiparametric Immunofluorescence Analysis

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Antibodies were directed to Ki-67 (AB16667, Abcam), calponin (SC136987, Santa Cruz), BECN1 (sc-11427, Santa Cruz), LC3B (#3868, Cell Signaling), LAMP1 (sc-17768, Santa Cruz), F4/80 (ab6640, Abcam), CD11b (PA5-18727, Invitrogen), Sca1 (ab-51317, Abcam), Osteopontin (ab8488, Abcam), LC3A/B (ab58610, Abcam). Secondary antibodies for immunofluorescence were Alexa Green 488 nm and Alexa Orange-Red 546 nm (Invitrogen). 4,6-diamidino-2-phenylindole (DAPI) (D3571), Prolong Gold Anti-fade mounting reagent (P36930), Slow-Fade Anti-fade reagent (S2828). Osteopontin was obtained from SIGMA (Cat. # O2260).

Pestell T.G., Jiao X., Kumar M., Peck A.R., Prisco M., Deng S., Li Z., Ertel A., Casimiro M.C., Ju X., Di Rocco A., Di Sante G., Katiyar S., Shupp A., Lisanti M.P., Jain P., Wu K., Rui H., Hooper D.C., Yu Z., Goldman A.R., Speicher D.W., Laury-Kleintop L, & Pestell R.G. (2017). Stromal cyclin D1 promotes heterotypic immune signaling and breast cancer growth. Oncotarget, 8(47), 81754-81775.

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Quantifying ECM Protein-Antibody Interactions

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Ninety-six-well ELISA plates (Greiner Bio-One) were coated with recombinant human ECM proteins (10 mg/ml), fibronectin (Sigma-Aldrich), fibrinogen (von Willebrand factor-and fibronectin-depleted; Enzyme Research Laboratories), osteopontin (Sigma-Aldrich), vitronectin (Sigma-Aldrich), collagen I (EMD Millipore), collagen II (EMD Millipore), collagen III (EMD Millipore), or collagen IV (EMD Millipore) in PBS for 1 hour at 37°C, followed by blocking with 2% BSA in PBS with 0.05% Tween 20 (PBS-T) for 1 hour at room temperature. Then, wells were washed with PBS-T and further incubated with PlGF-2 123-144 -Abs or unmodified Abs (10 mg/ml each) for 1 hour at room temperature. After three washes with PBS-T, wells were incubated for 1 hour at room temperature with horseradish peroxidase (HRP)-conjugated Abs against rat IgG or hamster IgG (Jackson ImmunoResearch). After washes, bound Abs were detected with tetramethylbenzidine sub-strate by measurement of the absorbance at 450 nm with subtraction of the absorbance at 570 nm.

Ishihara J., Fukunaga K., Ishihara A., Larsson H.M., Potin L., Hosseinchi P., Galliverti G., Swartz M.A, & Hubbell J.A. (2017). Matrix-binding checkpoint immunotherapies enhance antitumor efficacy and reduce adverse events. Science translational medicine, 9(415).

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