Phospho p65 p p65

Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), dimethylsulfoxide (DMSO), penicillin, and streptomycin were obtained from Gibco-BRL (Grand Island, NY, USA). Glyoxal solution (GO), sodium azide, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenylter-thiazolium bromide (MTT) were obtained from Sigma (St. Louis, MO, USA). ELISA kits (IL-6 and TNF-α) were from R&D Systems Inc. (Minneapolis, MN, USA). PRO-PREP™ protein extraction solution, and enhanced chemiluminescence (ECL) detection kits were availed from Intron (Sungnam, Korea). Antibodies against GAPDH, RAGE, IL 1 β, COX2, SIRT1, MMP-1, Type I Procollagen (COL1A), P38, phospho-P38 (p-P38), P65, phospho-P65 (p-P65), and secondary antibodies conjugated to horseradish peroxidase were purchased from Santa Cruz (Dallas, TX, USA), Cell Science (Canton, MA, USA), and Cell Signaling Technology (Beverly, MA, USA).

Dulbecco’s Modified Eagle’s Medium (DMEM), fetal bovine serum (FBS), penicillin-streptomycin, and phosphate-buffered saline (PBS) for cell culture were purchased from Gibco-BRL (Grand Island, NY, USA). Lipopolysaccharide (LPS), thiazolyl blue tetrazolium bromide (MTT), Griess reagent, sodium nitrite solution, the 19 selected volatile compound standards (α-pinene, camphene, β-pinene, sabinene, 3-carene, β-myrcene, α-phellandrene, α-terpinene, limonene, p-cymene, terpinolene, 2-nonanone, acetic acid, citronellal, bornyl acetate, thujopsene, β-chamigrene, (-)-β-bisabolene, and nerolidol) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). In addition, 2′,7′-dichlorodihydrofluorescein diacetate (H₂DCFDA) was obtained from Thermo Fisher Scientific (Carlsbad, CA, USA). Antibodies against iNOS, p65, phospho-p65 (p-p65), and β-actin were obtained from Santa-Cruz Biotechnology (Dallas, TX, USA). Materials for western blot were obtained from Bio-Rad (Hercules, CA, USA), and all other reagents and chemicals used were of analytical grade.