The platycodon secondary saponins (PSS) GP-682 and GPA-696 used in this work were synthesized in our laboratory. N,N-dimethylformamide (DMF) was purchased from Concord Technology Co., Ltd. (Tianjin, China). Nile Red, 9-diethylamino-5H-benzo[alpha]phenoxazine-5-one, was purchased from Aladdin (Beijing, China). FITC was purchased from MedChemExpress (New Jersey, USA). The Cytotoxicity Detection Kit (LDH Activity) was purchased from Roche (Basel, Switzerland). Glutaraldehyde (2.5%) was purchased from GenMed Scientific Inc. (MA, USA). Lev, one of the broad-spectrum antibiotics of quinolone, and terazosin hydrochloride were purchased from Shanghaiyuanye Bio-Technology Co., Ltd. (Shanghai, China). The Pseudomonas aeruginosa PA 14 strain was obtained from associate professor Bai Fang of Nankai University (Tianjin, China). A Pseudomonas aeruginosa antibody (1001/214) [Alexa Fluor® 488] was purchased from Bio-Techne China Co. Ltd. (Shanghai, China). The concentrations of human IL-6, IL-8 and TNF-α were detected using ELISA kits according to the manufacturer's instructions (Lanpai, Shanghai, China). All cell culture reagents were purchased from Gibco BRL Life Technologies (NY, USA).
Cytotoxicity detection kit ldh activity
Manufactured by Roche
Sourced in Switzerland
The Cytotoxicity Detection Kit (LDH Activity) is a laboratory tool designed to measure the release of lactate dehydrogenase (LDH) from damaged or lysed cells. LDH is a stable cytoplasmic enzyme that is released into the cell culture medium upon cell lysis or membrane damage, and this kit provides a quantitative assay to determine the amount of LDH activity as an indicator of cytotoxicity or cell death.
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3 protocols using cytotoxicity detection kit ldh activity
1
Platycodon Secondary Saponins Synthesis
2
Platycodon Secondary Saponin Antibacterial Effects
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The platycodon secondary saponins (PSS) GP-682 and GPA-696 used in this work were synthesized in our laboratory. N,N-dimethylformamide (DMF) was purchased from Concord Technology Co., Ltd. (Tianjin, China). Nile Red, 9-diethylamino-5H-benzo[alpha]phenoxazine-5-one, was purchased from Aladdin (Beijing, China). FITC was purchased from MedChemExpress (New Jersey, USA). The Cytotoxicity Detection Kit (LDH Activity) was purchased from Roche (Basel, Switzerland). Glutaraldehyde (2.5%) was purchased from GenMed Scientific Inc. (MA, USA). Lev, one of the broad-spectrum antibiotics of quinolone, and terazosin hydrochloride were purchased from Shanghaiyuanye Bio-Technology Co., Ltd. (Shanghai, China). The Pseudomonas aeruginosa PA 14 strain was obtained from associate professor Bai Fang of Nankai University (Tianjin, China).
A Pseudomonas aeruginosa antibody (1001/214) [Alexa Fluor® 488] was purchased from Bio-Techne China Co. Ltd. (Shanghai, China). The concentrations of human IL-6, IL-8 and TNF-α were detected using ELISA kits according to the manufacturer's instructions (Lanpai, Shanghai, China). All cell culture reagents were purchased from Gibco BRL Life Technologies (NY, USA).
A Pseudomonas aeruginosa antibody (1001/214) [Alexa Fluor® 488] was purchased from Bio-Techne China Co. Ltd. (Shanghai, China). The concentrations of human IL-6, IL-8 and TNF-α were detected using ELISA kits according to the manufacturer's instructions (Lanpai, Shanghai, China). All cell culture reagents were purchased from Gibco BRL Life Technologies (NY, USA).
3
Synthesis and Characterization of Platycodon Secondary Saponins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The platycodon secondary saponins (PSS) of GP-682 and GPA-696 used in this work were synthesized in our laboratory. N, N-dimethylformamide (DMF) was purchased from Concord Technology Co., Ltd.
(Tianjin, China). Nile Red, 9-diethylamino-5H-benzo [alpha] phenoxazine-5-one, was purchased from Aladdin (Beijing, China). FITC was purchased from MedChemexpress (New Jersey, USA). The Cytotoxicity Detection Kit (LDH Activity) was purchased from Roche (Basel, Switzerland). Glutaraldehyde (2.5%) was purchased from GenMed Scienti c Inc. (MA, USA). Lev, one of the broad-spectrum antibiotics of quinolone, and terazosin hydrochloride were purchased from Shanghaiyuanye Bio-Technology Co., Ltd.
(Shanghai, China). The Pseudomonas aeruginosa PA 14 strain was obtained from associate professor Bai Fang of Nankai University (Tianjin, China). Pseudomonas aeruginosa antibody (1001/214) [Alexa Fluor® 488] was purchased from Bio-Techne China Co. Ltd. (Shanghai, China). The concentrations of human IL-6, IL-8 and TNF-α were detected using ELISA kits according to the manufacturer's instructions (Lanpai, Shanghai, China). All cell culture reagents were purchased from Gibco BRL Life Technologies (NY, USA).
(Tianjin, China). Nile Red, 9-diethylamino-5H-benzo [alpha] phenoxazine-5-one, was purchased from Aladdin (Beijing, China). FITC was purchased from MedChemexpress (New Jersey, USA). The Cytotoxicity Detection Kit (LDH Activity) was purchased from Roche (Basel, Switzerland). Glutaraldehyde (2.5%) was purchased from GenMed Scienti c Inc. (MA, USA). Lev, one of the broad-spectrum antibiotics of quinolone, and terazosin hydrochloride were purchased from Shanghaiyuanye Bio-Technology Co., Ltd.
(Shanghai, China). The Pseudomonas aeruginosa PA 14 strain was obtained from associate professor Bai Fang of Nankai University (Tianjin, China). Pseudomonas aeruginosa antibody (1001/214) [Alexa Fluor® 488] was purchased from Bio-Techne China Co. Ltd. (Shanghai, China). The concentrations of human IL-6, IL-8 and TNF-α were detected using ELISA kits according to the manufacturer's instructions (Lanpai, Shanghai, China). All cell culture reagents were purchased from Gibco BRL Life Technologies (NY, USA).
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