Mouse monoclonal anti bcl 2

Manufactured by Santa Cruz Biotechnology

Sourced in United States

The Mouse monoclonal anti-Bcl-2 is a laboratory reagent that recognizes the Bcl-2 protein. Bcl-2 is an anti-apoptotic protein involved in the regulation of programmed cell death. This product can be used to detect and study the Bcl-2 protein in various experimental systems.

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Lab products found in correlation

Mouse monoclonal anti β actin, by Merck Group (2 mentions) Rabbit polyclonal anti cleaved caspase 3, by Cell Signaling Technology (1 mentions) Rabbit monoclonal anti parp, by Cell Signaling Technology (1 mentions) Annexin 5 fitc pi apoptosis kit, by BestBio (1 mentions) Reactive oxygen species assay kit, by Beyotime (1 mentions) Cck 8, by BestBio (1 mentions) Rabbit polyclonal anti caspase 3, by BD (1 mentions) Mouse monoclonal antibody against α sma, by Merck Group (1 mentions) Mouse monoclonal antibody against β actin, by Merck Group (1 mentions) Rabbit anti lc3 polyclonal antibody, by Novus Biologicals (1 mentions) Mouse monoclonal anti bax, by Santa Cruz Biotechnology (1 mentions) C digit blot scanner, by LI COR (1 mentions) Ripa lysis buffer, by Merck Group (1 mentions) Mouse anti β actin monoclonal antibody, by Santa Cruz Biotechnology (1 mentions) Immobilon western chemiluminescent hrp substrate, by Merck Group (1 mentions) Bovine serum albumin (bsa), by HiMedia (1 mentions) Anti akt, by Cell Signaling Technology (1 mentions) Anti gsk3β, by Cell Signaling Technology (1 mentions) Anti bcl xl, by Santa Cruz Biotechnology (1 mentions) Mouse anti ki 67, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

Bcl-2 (950 citations) Anti-Bcl-2 (395 citations) Mouse anti-Bcl-2 (31 citations) Monoclonal anti-BCL-2 (4 citations) Monoclonal mouse anti (2 citations)

7 protocols using mouse monoclonal anti bcl 2

Comprehensive Antibody Panel for Protein Analysis

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We used the following antibodies: mouse monoclonal anti-COMMD1 (clone 3B3; Abcam; ab131597), rabbit polyclonal anti-COMMD1 (Proteintech Group; 11938-1-AP), mouse monoclonal anti-β-Actin (Sigma-Aldrich; A5441), rabbit polyclonal anti-Tubulin (Abcam; ab4047), rabbit polyclonal anti-Lamin A/C (Cell Signaling Technology; #2032), mouse monoclonal anti-Bcl2 (Santa Cruz Biotechnology; sc-509), rabbit monoclonal anti-PARP (Cell Signaling Technology; #9532), rabbit polyclonal anti-cleaved-caspase3 (Cell Signaling Technology; #9661), mouse monoclonal anti-phospho-Ser10-Histone H3 antibody, (Cell Signaling Technology; #9706), mouse monoclonal anti-XIAP (BD Biosciences, # 610716), rabbit polyclonal anti-BRCA1 (Cell Signaling Technology; #9010), mouse monoclonal anti-Hsp90 (AC88) (Enzo; ADI-SPA-830), rabbit monoclonal anti-phospho-Ser139-H2AX (Cell Signaling Technology; #9718), Alexa-488-conjugated polyclonal anti-mouse (Molecular Probes; A-11001), Alexa-647-conjugated polyclonal anti-rabbit (Molecular Probes, A-21244), HRP-conjugated polyclonal goat anti-rabbit IgG (H + L) (Bio-Rad; #170–6515), HRP-conjugated polyclonal goat anti-mouse IgG (H + L) (Bio-Rad; #170–6516).

Fedoseienko A., Wieringa H.W., Wisman G.B., Duiker E., Reyners A.K., Hofker M.H., van der Zee A.G., van de Sluis B, & van Vugt M.A. (2016). Nuclear COMMD1 Is Associated with Cisplatin Sensitivity in Ovarian Cancer. PLoS ONE, 11(10), e0165385.

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Aucubin Modulates IL-1β-Induced Apoptosis

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Aucubin was purchased from Yuanmu Industrial Corporation (Shanghai, China). CCK-8 and Annexin V-FITC/PI Apoptosis kit were obtained from Bestbio (Shanghai, China). A Live and Dead Cell staining kit was purchased from Mountain View (CA, USA). A Reactive Oxygen Species Assay kit was purchased from Beyotime Biotechnology (Jiangsu, China). The primary antibodies used in this study were as follows: mouse monoclonal anti-Bcl-2 and monoclonal anti-Bax from Santa Cruz Biotechnology, Inc. (CA, USA), mouse monoclonal anti-cleaved caspase-3 and mouse monoclonal anti-cleaved caspase-9 from Abcam Biotechnology (MA, US). Recombinant Rat IL-1beta was obtained from PeproTech (NJ, USA).

Wang B.W., Jiang Y., Yao Z.L., Chen P.S., Yu B, & Wang S.N. (2019). Aucubin Protects Chondrocytes Against IL-1β-Induced Apoptosis In Vitro And Inhibits Osteoarthritis In Mice Model. Drug Design, Development and Therapy, 13, 3529-3538.

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Immunohistochemical Analysis of Apoptosis Markers

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Immunohistochemical staining of activated caspase-3 and Bcl-2 was performed as previously described (Ibrahim et al., 2015 (link)). The heart sections were deparafinized, rehydrated and blocked in 3% hydrogen peroxide. Then, the sections were incubated with rabbit polyclonal anti-caspase-3 (1:1000 dilution; BD Biosciences, Le Pont-de-Claix, France) and mouse monoclonal anti-Bcl-2 (1:200 dilution, Santa Cruz Biotechnology) as primary antibodies. Diaminobenzidine (DAB) chromogen was added to visualize the immune reaction. Finally, the slides were counterstained in hematotoxylin and cover-slipped. Semi quantitative grading score for assessment of activated caspase-3 and Bcl-2 was carried out, in ten random high power fields, according to the percentage of immune stained cells in high power field (HPF) (40X). Score 0 = no staining; 1-positive staining in < 25% of cells/HPF; 2-positive staining in 25–50% of cells/HPF; 3-positive staining in >50% of cells/HPF.

El-Marasy S.A., El Awdan S.A., Hassan A, & Abdallah H.M. (2020). Cardioprotective effect of thymol against adrenaline-induced myocardial injury in rats. Heliyon, 6(7), e04431.

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Immunoblotting and Immunohistochemistry Protocol

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The following antibodies were used for immunoblotting and/or immunohistochemistry: mouse monoclonal anti-beclin 1 (Santa Cruz Biotechnology, Santa Cruz, CA), mouse monoclonal anti-BCL2 (Santa Cruz Biotechnology, Santa Cruz, CA), rat monoclonal anti-Klotho antibody (KM2076) (TransGenics, Kobe, Japan), rabbit polyclonal anti-LC3 antibody (Novus Biologicals, Littleton, CO), mouse monoclonal antibody against α-SMA (Sigma Aldrich, St. Louis, MO), mouse monoclonal anti-p62/SQSTM1 antibody (Abnova, Taiwan), mouse monoclonal antibody against β-actin (Sigma Aldrich, St. Louis, MO), and mouse monoclonal antibody against glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Sigma Aldrich, St. Louis, MO). Rabbit polyclonal antibodies against human NaPi-2a or NaPi-2c were made by Yenzym Antibodies, LLC (San Francisco, CA). The peptide sequences for immunizing rabbit for NaPi-2a and NaPi-2c are human NaPi-2a peptide (77–96, CKLALEEEQKPESRLVPKLRQ) or human NaPi2c peptide (579–599, KATTKEAYCYENPEILASQQL) respectively and the specificity of anti-serum was confirmed with kidney tissues from NaPi-2a or 2c knockout mice. Secondary Abs coupled to horseradish peroxidase for immunoblotting, or to FITC, Alexa Fluor, or Cy5, and Syto 61 red fluorescent nuclear acid for immunohistochemistry were purchased from Molecular Probes/Invitrogen (Molecular Probes Inc., Eugene, OR).

Shi M., Maique J., Shaffer J., Davidson T., Sebti S., Fernández Á.F., Zou Z., Yan S., Levine B., Moe O.W, & Hu M.C. (2020). The Tripartite Interaction of Phosphate, Autophagy and αKlotho in Health Maintenance. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(2), 3129-3150.

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Protein Expression Analysis in THP-1 Cells

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Proteins were extracted from treated and non-treated THP-1 cells using radio-immunoprecipitation assay (RIPA) Lysis Buffer (Millipore, Burlington, MA, USA) following the were detected in total lysates from cell line cultures by Western Blotting. Membranes were incubated overnight at 4°C with these antibodies: goat polyclonal anti TfR1 (1:1000, Thermofisher), rabbit polyclonal anti ferroportin (1:1000, NOVUS), mouse monoclonal anti BAX (1:100, Santa Cruz), mouse monoclonal anti Bcl-2 (1:200, Santa Cruz), rabbit polyclonal anti Caspase 3 (1:2000, Bethyl Laboratories, Inc.), rabbit monoclonal anti pCDK2 (1:1000, abcam), rabbit polyclonal anti NFkB (1:500, Bethyl Laboratories, Inc.). Reactive bands were detected by chemiluminescence (Immobilon Western Chemiluminescent HRP Substrate, Millipore, Burlington, MA, USA) on a C-DiGit^® Blot Scanner (LI-COR Biotechnology^®, Lincoln, NE, USA). A mouse monoclonal anti β-Actin antibody (1:100, Santa Cruz) was used to check for comparable protein loading and as a housekeeping protein. Images were captured, stored and analyzed using “Image studio Digits ver. 5.0” software.

Argenziano M., Tortora C., Paola A.D., Pota E., Martino M.D., Pinto D.D., Leva C.D, & Rossi F. (2021). Eltrombopag and its iron chelating properties in pediatric acute myeloid leukemia. Oncotarget, 12(14), 1377-1387.

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Immunoblot and Immunohistochemical Analysis of Signaling Pathways

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For immunoblot and immunohistochemical analysis, various antibodies like rabbit monoclonal anti-p-MAPK, anti-MAPK, anti-p-Akt (Ser & Thr) and anti-Akt, anti-p-GSK-3β, anti-GSK-3β, anti-p-FoxO3a, anti-FoxO3a monoclonal mouse anti-CD-31, rabbit anti-caspase 3/7 were purchased from Cell Signaling Technology, Beverly, MA, USA. Mouse anti-Ki-67, rabbit monoclonal anti-PARP, mouse monoclonal anti-Bcl-2, anti-Bax, anti-Mcl-1, anti-Bcl-xL, HRP conjugated goat anti-rabbit IgG and goat anti-mouse IgG were purchased from Santa Cruz Biotechnology, Santa Cruz, CA, USA. Mouse monoclonal anti-β-actin and other reagents were procured from Sigma Aldrich, St. Louis, MO, USA. Antibodies dilutions have been made as per the manufacturer’s instruction. The pcDNA3-Akt-HA plasmid was obtained as gift sample from Dr. Guy Salvesen (University of California, San Diego, CA, USA). siRNA Akt and pcDNA3.1(-) were purchased from Cell Signaling Technology, Beverly, MA, USA and Invitrogen, USA respectively. Opti-MEM^®I Reduced Serum Media and fetal bovine serum (FBS) were purchased from Gibco-BRL Invitrogen Corporation, CA, USA. FuGENE® HD transfection reagent was obtained from Roche Applied Science, Mannheim, Germany. Trypsin, Bovine serum albumin (BSA) and antibiotics (10,000 U/L penicillin and 10 mg/L streptomycin) were purchased from Himedia, Mumbai, India.

Dey G., Bharti R., Dhanarajan G., Das S., Dey K.K., Kumar B.N., Sen R, & Mandal M. (2015). Marine lipopeptide Iturin A inhibits Akt mediated GSK3β and FoxO3a signaling and triggers apoptosis in breast cancer. Scientific Reports, 5, 10316.

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Western Blot Analysis of KSHV-Induced Signaling

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1×10 6 uninfected or KSHV-infected cells were lysed, subjected to electrophoresis and transferred to nitrocellulose membranes, as previously described (Gilardini Montani et al., 2018a) . Membranes were blocked in PBS-0.1% Tween 20 solution containing 3% BSA, probed with specific antibodies and developed using ECL Blotting Substrate (Advansta). The following antibodies were used: rabbit polyclonal anti-PARP1 (1:500; Cell Signaling Technology, 9542), mouse monoclonal anti-catalase (1:100; Santa Cruz Biotechnology, sc-271803), rabbit polyclonal anti-p-JNK (1:300; Cell Signaling, 4668), mouse monoclonal anti-JNK (1:100; Santa Cruz Biotechnology sc-7345), mouse monoclonal anti-p-Bcl2 (1:100; Santa Cruz Biotechnology sc-293128), mouse monoclonal anti-Bcl2 (1:100; Santa Cruz Biotechnology sc-7382), rabbit polyclonal anti-LC3B (1:1000; Novus Biologicals, NB100-2220SS), mouse monoclonal anti-SQSTM1 (1:500; BD Transduction Laboratories, 610883), rabbit polyclonal anti-ATG5 (1:500; Cell Signaling Technology, 2630), and anti-beta actin (1:10000; Sigma Aldrich, A5441). Goat anti-mouse IgG-HRP and anti-rabbit IgG-HRP (1:10.000 Santa Cruz Biotechnology Inc) were used as secondary antibodies.

Gilardini Montani M.S., Falcinelli L., Santarelli R., Romeo M.A., Granato M., Faggioni A, & Cirone M. (2019). Kaposi Sarcoma Herpes Virus (KSHV) infection inhibits macrophage formation and survival by counteracting Macrophage Colony-Stimulating Factor (M-CSF)-induced increase of Reactive Oxygen Species (ROS), c-Jun N-terminal kinase (JNK) phosphorylation and autophagy. The international journal of biochemistry & cell biology, 114.

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