Antibodies to bcl 2

Silver nitrate (AgNO₃) and MTT were obtained from Sigma-Aldrich (St Louis, MO, USA). Dried longan (Dimocarpus longan Lour.) was purchased from the Panyu Chinese Herbal Medicine Market in Guangzhou. Prostate cancer VCaP, pancreas cancer BxPC-3, and lung cancer H1299 cells were obtained from American Type Culture Collection (ATCC, Rockville, MD, USA). RPMI-1640 culture medium, fetal bovine serum (FBS), L-glutamine, and penicillin–streptomycin were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Trypan blue stain was obtained from Cambrex (Walkersville, MD, USA). Antibodies to β-actin, p-stat3, and survivin were purchased from EMD Millipore (Billerica, MA, USA) and antibodies to Bcl-2 from Santa Cruz Biotechnology Inc. (Dallas, TX, USA). Secondary antibodies were obtained from Santa Cruz Biotechnology. SuperSignal West Femto Luminol/Enhancer Solution was purchased from Thermo Fisher Scientific.