SW982 cells were seeded into a confocal culture dish (NEST Biotechnology Co., Ltd; Jiangsu, China) at a density of 5 × 105 cells per dish. 24 h later, the cells were pre-treated with SGE for 1 h then stimulated with IL-1β (20 ng/mL) for 1 h. Then the cell staining was conducted as previously [11 ].
Confocal culture dish
Manufactured by NEST Biotechnology
Sourced in China
The Confocal culture dish is a specialized laboratory equipment used for cell culture and microscopic imaging. It is designed to provide a controlled environment for growing and observing cells under a confocal microscope. The dish features a transparent bottom and walls, allowing for high-resolution imaging and analysis of cellular structures and processes.
Automatically generated - may contain errors
2 protocols using confocal culture dish
1
SW982 Cell Cytokine Stimulation Assay
2
Cellular Uptake of ApoEVs-AT
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For cellular uptake, 5×104 HFFs or HUVECs were first seeded into a confocal culture dish (NEST, China). Then, 100 μg ApoEVs -AT, suspended in 1 mL α-MEM, were labeled with 1 μg membrane-labeling dye DiO (Life Tech, V22886) at 37°C for 30 min, re-purified with the centrifugation at 16,000 g for 30 min at 4°C. HFFs or HUVECs were cultured with DiO-labeled ApoEVs-AT for 0, 4, and 8 h. Cells were fixed in 4% neutral paraformaldehyde (Biosharp, USA) at room temperature for 10 min and underwent permeabilization with 0.05% Triton X-100 (Sigma-Aldrich, USA) at room temperature for 15 min. The cytoskeleton was stained with phalloidin (1:200, Invitrogen, A34055-300U) at room temperature for 20 min and the nuclei were stained with DAPI (1:1000, Solarbio, C0050) at room temperature for 5 min. Images were captured by confocal microscopy (Olympus FV1000, Japan).
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