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Sc 7298

Manufactured by Abcam
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Sc-7298 is an antibody product offered by Abcam. It is a primary antibody, but no further details about its specifics can be provided without risk of extrapolation or interpretation.

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Lab products found in correlation

Ab14705, by Abcam (1 mentions) Ab110412, by Abcam (1 mentions) Ab14714, by Abcam (1 mentions) Ab77924, by Abcam (1 mentions) Anti pink1, by Novus Biologicals (1 mentions) Anti hsc70, by Santa Cruz Biotechnology (1 mentions) Anti parkin, by Abcam (1 mentions) Anti lamp1, by Cell Signaling Technology (1 mentions) Bc100 494, by Abcam (1 mentions) Anti p62, by Abnova (1 mentions) Anti lc3 1 2, by Novus Biologicals (1 mentions) Nb100 2220, by Cell Signaling Technology (1 mentions) Ab8245, by Abcam (1 mentions) Anti gapdh, by Abcam (1 mentions) Ripa buffer, by G Biosciences (1 mentions) Intercept blocking buffer, by LI COR (1 mentions) Odyssey m imager, by LI COR (1 mentions) Protease inhibitor cocktail, by Roche (1 mentions) Trans blot turbo transfer system, by Bio-Rad (1 mentions) Ab1790, by Abcam (1 mentions)

3 protocols using sc 7298

1

Western Blot Analysis of OXPHOS Proteins

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Antibodies anti‐COX4, anti‐UQCRC2, anti‐NDUFA9, anti‐ATP5A, anti‐SDHA were from Abcam (OXPHOS cocktail ab110412, dilution 1:2,000); anti‐SDHB (ab14714, dilution 1:200) and anti‐COX1 (ab14705, dilution 1:2,000) were from Abcam; anti‐HSC70 was from Santa Cruz (sc‐7298, dilution 1:1,000); anti‐GAPDH was from Abcam (ab8245, dilution 1:40,000); anti‐P62 was from Abnova (H00008878‐M01, dilution 1:1,000); anti‐LC3‐I/II was from Novus Biologicals (NB100‐2220, dilution 1:1,000); anti‐LAMP1 was from Cell Signaling (3243, dilution 1:1,000); anti‐PINK1 was from Novus (BC100‐494, dilution 1:1,000); and anti‐Parkin was from Abcam (ab77924, dilution 1:500).
Civiletto G., Dogan S.A., Cerutti R., Fagiolari G., Moggio M., Lamperti C., Benincá C., Viscomi C, & Zeviani M. (2018). Rapamycin rescues mitochondrial myopathy via coordinated activation of autophagy and lysosomal biogenesis. EMBO Molecular Medicine, 10(11), e8799.
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2

Western Blot Analysis of Protein Expression

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Cells were lysed in ice cold RIPA buffer (G-Biosciences, 786–490) supplemented with protease inhibitor cocktail (Roche, 11836153001). Samples were denatured, separated by SDS-PAGE with 4–12% Bis-tris gels (Sigma), and transferred to low fluorescence PVDF membranes with the Trans-Blot Turbo Transfer System according to manufacturer’s instructions (Bio-Rad, RRID:SCR_023156). Membranes were blocked for one hour with Intercept Blocking Buffer (LI-COR, 927–7000) and probed overnight at 4°C with the following primary antibodies diluted in blocking buffer containing 0.2% Tween-20: Puromycin (1:10000, Millipore MABE343, RRID:AB_2566826), APOE (1:1000, GeneTex GTX100053, RRID:AB_1949674), HSC70 (1:1000, Santa Cruz sc-7298, RRID:AB_627761), LRP1 (1:50000, abcam 92544, RRID:AB_2234877). Membranes were washed with PBST and incubated for an hour with IRDye 680RD goat anti-mouse (LI-COR 926–68070, RRID:AB_10956588) or 800CW goat anti-rabbit (LI-COR 926–32211, RRID:AB_621843) secondary antibodies diluted in blocking buffer containing 0.2% Tween 20 and 0.1% SDS (1:5000). Blots were imaged with a LI-COR Odyssey M Imager and analyzed with Image Studio Lite (RRID:SCR_013715) and Empiria Studio (RRID:SCR_022512) software.
Antibody-targeted superantigens are potent inducers of tumor-infiltrating T lymphocytes in vivo. (1995). Proceedings of the National Academy of Sciences of the United States of America, 92(26), 12530.
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3

Western Blot Analysis of Nuclear Proteins

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Proteins were separated by SDS-PAGE on 4%-20% polyacrylamide gradient gels and transferred to 0.45-mm nitrocellulose membranes (Whatman Protran). The membranes were incubated with the appropriate primary and secondary antibodies and washed with TBS-Tween 20. Horseradish-peroxidase-conjugated secondary antibodies were detected by SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific PI-34080). Antibodies used were anti-SAP155 (MBL; D221-3), anti-H3 (Abcam; ab1791), anti-H2B (Abcam; ab1790), anti-SF3B4 (Abcam; ab11803), anti-SF3B3 (Proteintech; 14577), anti-SF3A1 (Proteintech; 15858), anti-SNRPA1 (Proteintech; 17368), anti-SNRPA (Santa Cruz; sc-376027), anti HSC-70 (Santa Cruz; sc-7298), donkey anti-rabbit (Abcam; ab97064), and goat anti-mouse immunoglobulin G (Abcam; ab7068).
Kfir N., Lev-Maor G., Glaich O., Alajem A., Datta A., Sze S.K., Meshorer E, & Ast G. (2015). SF3B1 association with chromatin determines splicing outcomes. Cell reports, 11(4).
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