All the standards (retinol, retinyl acetate, dl-α-tocopherol, α-toco-pheryl acetate and cholecalciferol), the isotope- labeled internal standards of cholecalciferol (6,19,19-d3 solution) and α-tocopherol (phenyl-5,7-dimethyl-d6)) as well as L-ascorbic acid, butylated hydroxyl-toluene (BHT), α-amylase from Aspergillus oryzae and sodium hydroxide were purchased from Sigma Aldrich (Steinheim, Germany). Methanol, ethanol, and Acetonitrile HPLC grade, and absolute ethanol and petroleum ether analytical grade, were obtained from Fischer Scientific (Geel, Belgium). Acetonitrile and Methanol LC-MS grade were acquired from Merck (Darmstadt, Germany), while anhydrous sodium sulfate was purchased from Honeywell (Offenbach, Germany). Distilled water was provided by a MilliQ purification apparatus (Millipore Direct-Q UV, Bedford, MA, USA). Chromafil Regenerated Cellulose (RC) syringe filters (15 mm diameter, 0.2 mm pore size) were obtained from Macherey-Nagel (Düren, Germany). Solid-phase extraction (SPE) Strata Silica 500 mg/6 mL cartridges (55 m, 70Å) were purchased from Phenomenex (Torrance, CA, USA).
Anhydrous sodium sulfate
Manufactured by Honeywell
Sourced in Germany
Anhydrous sodium sulfate is a white crystalline powder that is commonly used as a drying agent or desiccant in various laboratory applications. It is an inorganic salt with the chemical formula Na2SO4. Anhydrous sodium sulfate is highly effective in removing water from organic solvents and other liquids, making it a valuable tool for the purification and drying of chemicals and samples.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by Thermo Fisher Scientific (3 mentions)
Absolute ethanol, by Thermo Fisher Scientific (1 mentions)
Retinyl acetate, by Merck Group (1 mentions)
Hplc grade acetonitrile, by Thermo Fisher Scientific (1 mentions)
α tocopheryl acetate, by Merck Group (1 mentions)
Direct q uv, by Merck Group (1 mentions)
Dl α tocopherol, by Merck Group (1 mentions)
Lc ms grade methanol, by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
L ascorbic acid, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Acetyl chloride, by Merck Group (1 mentions)
C11 0 fame, by Merck Group (1 mentions)
N hexane, by Merck Group (1 mentions)
2 protocols using anhydrous sodium sulfate
1
Simultaneous Quantification of Fat-Soluble Vitamins
2
Seaweed Fatty Acids Methyl Esters Analysis
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Seaweed fatty acids methyl esters (FAMEs) were prepared according to the methodology previously described by Kumari et al. [16] . Briefly, to 500 mg of powdered dried sample was added a mixture of 5 mL of acetyl chloride (Sigma-Aldrich®, Germany) and methanol (Fisher Scientific, UK) reagent in a ratio of 1:19 (v/v), spiked with 10 µL of an internal standard solution (1 mg/mL, C11:0 FAME (Sigma-Aldrich®, Germany)/C13:0 TAG (Sigma-Aldrich®, USA) in n-hexane (Merck KGaA, Germany)). The solution was esterified at 80 °C for 1 hour and then left at room temperature for cooling. Next, 1 mL of water and 2 mL of n-hexane were added to the mixture, vortexed, and centrifuged at 2057 × g for 5 min. The organic phase was collected, filtered, and dried using anhydrous sodium sulfate (Honeywell, Germany). Finally, solvents were later removed under a nitrogen flow and methyl esters were solubilized in 190 µL of n-hexane for GC-MS analysis.
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