All p190A expression constructs were synthesized with an N-terminal Myc-tag by Gene Oracle, Inc. and cloned into the lentiviral vector pUltra-hot from Addgene, plasmid #24130. The entire cDNAs for wild-type or mutant p190A forms were verified by Sanger sequencing. Validated pLKO.1 puro shRNA E-cadherin lentiviral plasmid was purchased from Addgene, plasmid #18801. Validated pLenti-EmGFP-LATS2/1-kd plasmid to deplete LATS1 and LATS2 together was likewise obtained from Addgene, plasmid #52085. Lentiviral expression construct encoding mouse E-cadherin was similarly purchased from Addgene, plasmid #18804. Lentiviral pZIP vectors encoding shRNAs targeting human p190A were purchased from transOMIC technologies Inc; cat. no. TRHS1000–35 (ARHGAP35/p190A) and validated previously39 (link).
Validated plenti emgfp lats2 1 kd plasmid
Manufactured by Addgene
The validated pLenti-EmGFP-LATS2/1-kd plasmid is a lentiviral expression vector that allows for the expression of a GFP-tagged, kinase-dead mutant of the LATS2 protein. This plasmid can be used for the study of LATS2 signaling pathways in mammalian cell lines.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using validated plenti emgfp lats2 1 kd plasmid
1
Generating Lentiviral Constructs for p190A Overexpression and Knockdown
2
Lentiviral Constructs for p190A and E-cadherin
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All p190A expression constructs were synthesized with an N-terminal Myc-tag by Gene Oracle, Inc. and cloned into the lentiviral vector pUltra-hot from Addgene, plasmid #24130. The entire cDNAs for wild-type or mutant p190A forms were verified by Sanger sequencing. Validated pLKO.1 puro shRNA E-cadherin lentiviral plasmid was purchased from Addgene, plasmid #18801. Validated pLenti-EmGFP-LATS2/1-kd plasmid to deplete LATS1 and LATS2 together was likewise obtained from Addgene, plasmid #52085. Lentiviral expression construct encoding mouse E-cadherin was similarly purchased from Addgene, plasmid #18804. Lentiviral pZIP vectors encoding shRNAs targeting human p190A were purchased from transOMIC technologies Inc; cat. no. TRHS1000-35 (ARHGAP35/p190A) and validated previously [39 (link)].
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!