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Na 1.1 ld c apochromatic water immersion objective

Manufactured by Hamamatsu Photonics
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Sourced in Japan

The 40×/NA 1.1 LD C-Apochromatic water immersion objective is a high-performance microscope objective designed for use in scientific applications. It provides a high numerical aperture of 1.1, allowing for superior optical resolution and light-gathering capabilities. The objective is optimized for use with water as the immersion medium, making it suitable for applications that require aqueous sample environments. The C-Apochromatic design helps to minimize chromatic aberrations, ensuring high-quality, distortion-free images across a wide range of wavelengths.

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Lab products found in correlation

Orca flash 4.0 lt cmos digital camera, by Hamamatsu Photonics (2 mentions) Axio observer z1 7, by Zeiss (2 mentions)

2 protocols using na 1.1 ld c apochromatic water immersion objective

1

Measuring Cilium Length in Cells

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To measure cilium length, cells grown to mid-log phase were fixed and stained using 1 part Lugol’s solution (EMD Millipore Cat. No. 1.09261.1000) with 3 parts culture (usually 25 μl and 75 μl). 4 μl were loaded onto a slide, spread by placing a No. 1.5 coverslip on thee sample, and imaged coverslip slide down with a Zeiss Axio Observer.Z1/7 Widefield microscope with a Hamamatsu Orca-Flash 4.0 LT CMOS Digital Camera (Hamamatsu Photonics, Hamamatsu City, Japan) and 40×/NA 1.1 LD C-Apochromatic water immersion objective. Images were acquired with 10 ms exposure and 8.0 V of light intensity, using the PH3 phase contrast ring. Ciliary lengths were traced and measured in Fiji94 (link).
Coyle M.C., Tajima A.M., Leon F., Choksi S.P., Yang A., Espinoza S., Hughes T.R., Reiter J.F., Booth D.S, & King N. (2023). An RFX transcription factor regulates ciliogenesis in the closest living relatives of animals. Current biology : CB, 33(17), 3747-3758.e9.
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2

Measuring Cilium Length in Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
To measure cilium length, cells grown to mid-log phase were fixed and stained using 1 part Lugol’s solution (EMD Millipore Cat. No. 1.09261.1000) with 3 parts culture (usually 25 μl and 75 μl). 4 μl were loaded onto a slide, spread by placing a No. 1.5 coverslip on thee sample, and imaged coverslip slide down with a Zeiss Axio Observer.Z1/7 Widefield microscope with a Hamamatsu Orca-Flash 4.0 LT CMOS Digital Camera (Hamamatsu Photonics, Hamamatsu City, Japan) and 40×/NA 1.1 LD C-Apochromatic water immersion objective. Images were acquired with 10 ms exposure and 8.0 V of light intensity, using the PH3 phase contrast ring. Ciliary lengths were traced and measured in Fiji94 (link).
Lu M, & Kosik K.S. (2001). Competition for Microtubule-binding with Dual Expression of Tau Missense and Splice Isoforms. Molecular Biology of the Cell, 12(1), 171-184.
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