Sensifast probe hi rox

Manufactured by Meridian Bioscience

The SensiFAST Probe Hi-ROX is a real-time PCR master mix designed for use with probe-based detection systems. It provides fast and reliable amplification of DNA targets.

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Lab products found in correlation

Magmax 96 for microarrays total rna isolation kit, by Thermo Fisher Scientific (2 mentions) Superscript vilo master mix, by Thermo Fisher Scientific (2 mentions) Veriti 96 well pcrthermal cycler, by Thermo Fisher Scientific (2 mentions) Rnase free dnase set, by Qiagen (1 mentions) Ribonuclease free dnase set, by Qiagen (1 mentions)

Spelling variants of this product

SensiFAST Probe Hi-ROX Kit (32 citations) SensiFAST Probe Hi-ROX Mix (19 citations) SensiFAST Probe Hi-ROX One-Step kit (12 citations) SensiFAST™ probe HI-ROX master mix (4 citations) SensiFast Probe Hi-Rox reagent (3 citations) SensiFAST™ Probes (2 citations)

2 protocols using sensifast probe hi rox

Gene Expression Analysis via RT-qPCR

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Tissues were homogenized in RNALater and purified using MagMAX-96 for Microarrays Total RNA Isolation Kit (Ambion by Life Technologies). Genomic DNA was removed using RNase-Free DNase Set (Qiagen). mRNA was reverse-transcribed into cDNA using SuperScript VILO Master Mix (Invitrogen Life Technologies) and Veriti 96-well PCR Thermal Cycler (Thermo Fisher Scientific). cDNA was amplified with SensiFAST Probe Hi-ROX (Meridian Life Science) using 12k Flex System (Applied Biosystems). PCR reactions were done in triplicate. GAPDH was used to normalize cDNA input differences. Data reported as comparative CT method using delta delta CT. Probes listed in the Supplementary Materials.

Carbonaro M., Lee J., Pefanis E., Desclaux M., Wang K., Pennington A., Huang H., Mujica A., Rojas J., Ally R., Kennedy D., Brown M., Rogulin V., Moller-Tank S., Sabin L., Zambrowicz B., Thurston G, & Li Z. (2022). Efficient engraftment and viral transduction of human hepatocytes in an FRG rat liver humanization model. Scientific Reports, 12, 14079.

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TaqMan Real-Time PCR for Gene Expression

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TaqMan real-time PCR was performed as previously described with slight modifications (43 (link)). Tissues were homogenized in RNAlater and were purified using the MagMAX-96 for microarrays total RNA isolation kit (Ambion by Life Technologies). Genomic DNA was removed using a ribonuclease-free DNase set (QIAGEN). mRNA was reverse-transcribed into cDNA using the SuperScript VILO master mix (Invitrogen Life Technologies) and a Veriti 96-well PCR thermal cycler (Thermo Fisher Scientific). cDNA was amplified with SensiFAST Probe Hi-ROX (Meridian Life Science) using 12k Flex System (Applied Biosystems). PCR reactions were done in triplicate, and GAPDH was used to normalize cDNA input differences. Data are reported as relative quantification using ΔΔCt. Primer and probe sequences are listed in table S1.

Carbonaro M., Wang K., Huang H., Frleta D., Patel A., Pennington A., Desclaux M., Moller-Tank S., Grindley J., Altarejos J., Zhong J., Polites G., Poueymirou W., Jaspers S., Kyratsous C., Zambrowicz B., Murphy A., Lin J.C., Macdonald L.E., Daly C., Sleeman M., Thurston G, & Li Z. (2023). IL-6–GP130 signaling protects human hepatocytes against lipid droplet accumulation in humanized liver models. Science Advances, 9(15), eadf4490.

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