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Sp8confocal laser scanning platform

Manufactured by Leica
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The SP8 confocal laser scanning platform is a high-performance microscopy system designed for advanced imaging applications. It features a modular architecture that allows for customization to meet specific research needs. The SP8 platform provides users with the tools to capture detailed, high-resolution images of biological samples.

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Lab products found in correlation

Prolong gold antifade containing dapi, by Thermo Fisher Scientific (2 mentions) Apoxin green indicator and 1 7 aad apoptosis necrosis detection kit, by Abcam (2 mentions)

2 protocols using sp8confocal laser scanning platform

1

Quantifying Apoptosis and Necrosis in A549 Cells

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A549 lung epithelial cells were grown to confluency on fibronectin-coated circular glass coverslips in 24-well tissue culture plates and then incubated with 50 μg/ml (2.9 μM) mucoricin or 5 μg/ml (77 nM) ricin (concentrations shown to cause in vitro damage to alveolar epithelial cells [Fig. 3f]) for 2 hours after which the cells were washed and stained with 1x Apoxin Green Indicator and 1× 7-AAD (Apoptosis/Necrosis detection kit, Abcam) for 45 min. The cells were fixed and mounted in ProLong Gold antifade containing DAPI (Life Technologies) to visualize cells. Microscopic z-stack pictures were taken using a Leica SP8confocal laser scanning platform. Apoptotic cells vs. necrotic cells were identified by their green and red fluorescence, respectively. The number of apoptotic and necrotic events per high-power field (HPF) was determined, counting 10 HPF per coverslip. The experiment was performed three times in triplicate.
Soliman S.S., Baldin C., Gu Y., Singh S., Gebremariam T., Swidergall M., Alqarihi A., Youssef E.G., Alkhazraji S., Pikoulas A., Perske C., Venkataramani V., Rich A., Bruno V.M., Hotopp J.D., Mantis N.J., Edwards JE J.r., Filler S.G., Chamilos G., Vitetta E.S, & Ibrahim A.S. (2021). Mucoricin is a Ricin-Like Toxin that is Critical for the Pathogenesis of Mucormycosis. Nature microbiology, 6(3), 313-326.
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2

Quantifying Apoptosis and Necrosis in A549 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
A549 lung epithelial cells were grown to confluency on fibronectin-coated circular glass coverslips in 24-well tissue culture plates and then incubated with 50 μg/ml (2.9 μM) mucoricin or 5 μg/ml (77 nM) ricin (concentrations shown to cause in vitro damage to alveolar epithelial cells [Fig. 3f]) for 2 hours after which the cells were washed and stained with 1x Apoxin Green Indicator and 1× 7-AAD (Apoptosis/Necrosis detection kit, Abcam) for 45 min. The cells were fixed and mounted in ProLong Gold antifade containing DAPI (Life Technologies) to visualize cells. Microscopic z-stack pictures were taken using a Leica SP8confocal laser scanning platform. Apoptotic cells vs. necrotic cells were identified by their green and red fluorescence, respectively. The number of apoptotic and necrotic events per high-power field (HPF) was determined, counting 10 HPF per coverslip. The experiment was performed three times in triplicate.
Soliman S.S., Baldin C., Gu Y., Singh S., Gebremariam T., Swidergall M., Alqarihi A., Youssef E.G., Alkhazraji S., Pikoulas A., Perske C., Venkataramani V., Rich A., Bruno V.M., Hotopp J.D., Mantis N.J., Edwards JE J.r., Filler S.G., Chamilos G., Vitetta E.S, & Ibrahim A.S. (2021). Mucoricin is a Ricin-Like Toxin that is Critical for the Pathogenesis of Mucormycosis. Nature microbiology, 6(3), 313-326.
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