Anti il10r antibody

Helicobacter hepaticus was originally purchased from ATCC (#51449). Frozen stock was stored at −80°C in Brucella broth (BD) with 20% glycerol (Sigma-Aldrich). For maintenance and infection, the bacteria were grown on Brucella agar plates (BD) supplemented with 5% sheep blood (Thermo Scientific) in anaerobic jars with Oxoid CampyGen sachet (Thermo Scientific) at 37°C for 5 days. Mice were oral infected with 10⁸ CFU Helicobacter hepaticus on day 0 and 5, and 800 μg of anti-IL-10R antibody (BioXcell, clone 1B1.3A) was administered intraperitoneally per week. Tissue samples from the intestines of mice were fixed with 4% paraformaldehyde, embedded in paraffin, and 5 μm sections were stained with haematoxylin and eosin. Histopathological scoring of intestinal inflammation was performed in a blind manner according to a previous publication^{53 (link)}. Briefly, inflammation was graded on a scale from 0 to 3, including the following criteria: (i) epithelial hyperplasia and goblet cell depletion, (ii) lamina propria leukocyte infiltration, (iii) area of tissue affected, (iv) markers of severe inflammation, including crypt abscesses, sub-mucosal inflammation, and ulceration. Scores for individual criteria were added up for an overall inflammation score between 0 and 12.