Embryos were soaked in Tyrode’s solution containing BrdU (final concentration; 0.1 mM, Thermo Fisher, B23151) with or without aphidicolin (SIGMA, A0781) at 37 °C for 15 min, and cultured for 12 hr at 37 °C (BrdU was incorporated for 12 hr). Embryos were then fixed in 4% paraformaldehyde (PFA, Electron Microscopy Sciences)/PBS for 30 min at room temperature (RT). Embryos were then washed with PBS to remove PFA, and unincorporated BrdU, and incubated with 1 M HCl for 1 hr at RT to denature DNA. BrdU signal was detected by immunofluorescence staining with anti-BrdU antibody (1∶200, Millipore, MAB3424).
B23151
Manufactured by Merck Group
B23151 is a laboratory equipment product manufactured by Merck Group. It is designed to serve as a centrifuge for separation and purification applications in research and diagnostic settings. The core function of this product is to facilitate the separation of different components within a liquid sample through the application of centrifugal force.
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Lab products found in correlation
2 protocols using b23151
1
BrdU Incorporation and Aphidicolin Treatment
2
Quantifying Cell Proliferation via BrdU
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Embryos were soaked in Tyrode’s solution containing BrdU (final concentration; 0.1mM, Thermo Fisher, B23151) with or without aphidicolin (SIGMA, A0781) at 37°C for 15 minutes, and cultured for 16 hours at 37°C (BrdU was incorporated for 16 hours). Embryos were then fixed in 4% paraformaldehyde (PFA, Electron Microscopy Sciences)/PBS for 30 minutes at room temperature (RT). Embryos were then washed with PBS to remove PFA, and unincorporated BrdU, and incubated with 1M HCl for 1 hour at RT to denature DNA. BrdU signal was detected by immunofluorescence staining with anti-BrdU antibody (1.200, Millipore, MAB3424).
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