Human muscle satellite cells (hMuSCs; ScienCell Inc., Carlsbad, CA, USA) were cultured in growth media (DMEM containing 10% FBS, 10% HS, 0.5% chick embryo extract (CEE; MP Biomedicals, Santa Ana, CA, USA), and 1% P/S). On the day of cell seeding, hMuSCs were trypsinized and resuspended in low‐serum medium (DMEM containing 10% FBS and 1% P/S) at a concentration of 3 × 106 per mL, and seeded onto the muscle matrix derived from decellularized gastrocnemius muscles. After 3 or 7 days, hMuSC‐ECM cultures were fixed in 2% PFA for 20 min and transferred to 30% sucrose solution for 24 h. The fixed tissues were then snap‐frozen in liquid nitrogen‐cooled 2‐methylbutane. Samples were then cryosectioned (7 μm) and mounted on glass slides. Sections were incubated overnight with either rat anti‐Er‐Tr7 antibody (1:500; Hycult, Plymouth Meeting, PA, USA) or rabbit anti‐desmin antibody (1:1000; Abcam, Cambridge, MA, USA), followed by a 45‐min incubation with AlexaFluor 594 goat anti‐rat or AlexaFluor 488 goat anti‐rabbit secondary antibodies (1:1000; Life Technologies).
Rabbit anti desmin antibody
Manufactured by Abcam
Sourced in United States, United Kingdom
Rabbit anti-desmin antibody is a primary antibody that specifically binds to the desmin protein, which is a type III intermediate filament found in muscle cells. This antibody can be used in various applications, such as Western blotting, immunohistochemistry, and immunocytochemistry, to detect and analyze the presence and distribution of desmin in biological samples.
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2 protocols using rabbit anti desmin antibody
1
Characterizing Human Muscle Satellite Cells
2
Immunohistochemical Tissue Analysis
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Serial sections (5 μm) were cut from the paraffin blocks. Hematoxylin-eosin staining (HE) and immunohistochemistry staining (IHC) were performed. IHC was performed with Dako Target Retrieval Solution, Citrate pH 6 (10x) (Dako-Agilent Technologies, Santa Clara, CA, USA; Cat. No. S236784), Dako REAL Peroxidase-Blocking Solution (Cat. No. S2023), Protein Block Serum-Free Ready-to-use (Cat. No. X0909), Dako REAL Antibody Diluent (Cat. No. S2022) and Dako REAL EnVision Detection System, Peroxidase/3,3'-Diaminobenzidine +, Rabbit/Mouse (Cat. No. K5007). These procedures were performed according to Dako's protocol. After visualization with DAB, the sections were counter-stained with hematoxylin. The stained specimens were observed using an Olympus BX53 microscope (Olympus, Tokyo, Japan). The immunopositive areas were measured using WinROOF (Mitani).
The primary antibodies employed were as follows: Rabbit Anti-Desmin antibody (Abcam, Cambridge, UK; Cat. No. ab15200) 1:200, CD34 Monoclonal Antibody (QBEND/10) (Invitrogen-Thermo Fisher Scientific; Cat. No. MA1-10202) 1:500 and Platelet-derived growth factor (PDGF)-A Antibody (E−10: Santa Cruz, Dallas, TX, USA; Cat. No. sc-9974) 1:100.
The primary antibodies employed were as follows: Rabbit Anti-Desmin antibody (Abcam, Cambridge, UK; Cat. No. ab15200) 1:200, CD34 Monoclonal Antibody (QBEND/10) (Invitrogen-Thermo Fisher Scientific; Cat. No. MA1-10202) 1:500 and Platelet-derived growth factor (PDGF)-A Antibody (E−10: Santa Cruz, Dallas, TX, USA; Cat. No. sc-9974) 1:100.
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