Para n sections (5-µm) of the ileum tissue underwent appropriate heat-induced antigen retrieval and blocked as previously described ( 16). The following primary antibodies were used: rabbit anti-Notch1 antibody (Abcam, Shanghai, China), rabbit anti-beta Catenin antibody (Abcam), rabbit anti-Muc2 antibody (Proteintech, Wuhan, China), rabbit anti-Lyz1 antibody (Abcam) and rabbit anti-Chga antibody (Abcam). For negative controls, mouse or rabbit nonimmune serum, instead of the primary Abs, was used to treat tissue sections. Intensities quanti cation of Notch1, β-catenin, Muc2, Lyz1 and Chga staining was performed by automated image analysis in ve randomly chosen 200X elds of each sample.
Rabbit anti muc2 antibody
Manufactured by Proteintech
Rabbit anti-Muc2 antibody is a laboratory reagent that specifically recognizes the Muc2 protein. It can be used to detect and quantify Muc2 expression in various experimental systems.
Automatically generated - may contain errors
2 protocols using rabbit anti muc2 antibody
1
Immunohistochemical Analysis of Intestinal Markers
2
Immunohistochemical Analysis of Intestinal Markers
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Para n sections (5-µm) of the ileum tissue underwent appropriate heat-induced antigen retrieval and blocked as previously described ( 16). The following primary antibodies were used: rabbit anti-Notch1 antibody (Abcam, Shanghai, China), rabbit anti-beta Catenin antibody (Abcam), rabbit anti-Muc2 antibody (Proteintech, Wuhan, China), rabbit anti-Lyz1 antibody (Abcam) and rabbit anti-Chga antibody (Abcam). For negative controls, mouse or rabbit nonimmune serum, instead of the primary Abs, was used to treat tissue sections. Intensities quanti cation of Notch1, β-catenin, Muc2, Lyz1 and Chga staining was performed by automated image analysis in ve randomly chosen 200X elds of each sample.
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