Splenic CD11b+ cells were isolated using CD11b MicroBeads kit (Miltenyi Biotec, Catalog #:130-049-601). The CD11b+F4/80+ cells were isolated from CD11B+ cells by FACS sorting. Total RNAs were isolated with RNeasy mini kit (QIAGEN, Cat #: 74104). The total RNA was further processed for measuring RNA quality by RNA integrity number RIN), only RIN over 9 was accepted for further processing. 1 μg of total RNA was subjected to library synthesis using the TruSeq V2 RNA-Seq kit. Total RNA was enriched for the Poly-A mRNA and reverse transcribed to double-stranded cDNA. Sequencing was performed using 12pM hybridization to a 2x100 paired end flow cell by running standard illumine HiSeq4000 sequencing, and obtained > 50 million reads for each sample by the Genomics and Bioinformatics Core of Yale Stem Cell Center.
Cd11b microbeads kit
Manufactured by Miltenyi Biotec
The CD11b MicroBeads kit is a laboratory product designed for the isolation and enrichment of CD11b-positive cells from various sample sources, such as peripheral blood, bone marrow, or tissue samples. The kit contains magnetic beads coated with antibodies specific to the CD11b surface antigen, which is expressed on myeloid cells, including monocytes, macrophages, and granulocytes. This product enables the efficient separation and purification of CD11b-expressing cells using standard magnetic cell separation techniques.
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Lab products found in correlation
2 protocols using cd11b microbeads kit
1
Splenic CD11b+ Cell Isolation and RNA-Seq
2
Splenic CD11b+ Cell Isolation and RNA-Seq
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