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Shank1

Manufactured by Synaptic Systems
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Shank1 is a component used in research applications involving the study of neurological structures and processes. It serves as a specialized tool for precisely manipulating and positioning samples during microscopic analysis. The core function of Shank1 is to enable controlled and accurate positioning of specimens for detailed observation and investigation.

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Lab products found in correlation

Camkiia, by Merck Group (2 mentions) Homer1, by Merck Group (2 mentions) Homer3, by Synaptic Systems (2 mentions) Shank2, by Cell Signaling Technology (2 mentions) Shank3, by Santa Cruz Biotechnology (2 mentions) Glur1, by Merck Group (2 mentions) β actin, by Merck Group (2 mentions) Mglur5, by Abcam (2 mentions)

2 protocols using shank1

1

Protein analysis of synaptic fractions

Check if the same lab product or an alternative is used in the 5 most similar protocols
PSD and synaptosomal fractions of the striatum, cortex, and cerebellum were prepared as previously described21 (link). Purified fractions were separated on SDS-PAGE and quantified using Odyssey Licor. β-Actin and Tubulin were used as loading controls. Specific primary antibody for SAPAP3 was prepared as previously described21 (link). Commercial antibodies used include SHANK3 (Santa Cruz SC-30193), GluR1 (Millipore MAB2263), GluR2 (Neuromab 75-002), NR1 (BD Biosciences 556308), NR2A (Millipore 07-632), NR2B (Millipore 05-920), Homer1 (Chemicon AB5877, Synaptic Systems 160022), Homer3 (Synaptic Systems 160303), mGLUR5 (Abcam ab76316), CaMKIIa (Millipore 05-532), Shank1 (Synaptic Systems 162002), Shank2 (Cell Signaling 12218S), B-Actin (Sigma A5441), and Tubulin (Sigma T5168). Statistical analysis was done using two-tailed Students’ t-tests.
Mei Y., Monteiro P., Zhou Y., Kim J.A., Gao X., Fu Z, & Feng G. (2016). Adult Restoration of Shank3 Expression Rescues Selective Autistic-Like Phenotypes. Nature, 530(7591), 481-484.
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2

Protein analysis of synaptic fractions

Check if the same lab product or an alternative is used in the 5 most similar protocols
PSD and synaptosomal fractions of the striatum, cortex, and cerebellum were prepared as previously described21 (link). Purified fractions were separated on SDS-PAGE and quantified using Odyssey Licor. β-Actin and Tubulin were used as loading controls. Specific primary antibody for SAPAP3 was prepared as previously described21 (link). Commercial antibodies used include SHANK3 (Santa Cruz SC-30193), GluR1 (Millipore MAB2263), GluR2 (Neuromab 75-002), NR1 (BD Biosciences 556308), NR2A (Millipore 07-632), NR2B (Millipore 05-920), Homer1 (Chemicon AB5877, Synaptic Systems 160022), Homer3 (Synaptic Systems 160303), mGLUR5 (Abcam ab76316), CaMKIIa (Millipore 05-532), Shank1 (Synaptic Systems 162002), Shank2 (Cell Signaling 12218S), B-Actin (Sigma A5441), and Tubulin (Sigma T5168). Statistical analysis was done using two-tailed Students’ t-tests.
Mei Y., Monteiro P., Zhou Y., Kim J.A., Gao X., Fu Z, & Feng G. (2016). Adult Restoration of Shank3 Expression Rescues Selective Autistic-Like Phenotypes. Nature, 530(7591), 481-484.
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